組蛋白伴侶FACT識(shí)別組蛋白H2A 和 H2B，在轉(zhuǎn)錄,、復(fù)制和DNA修復(fù)過程中有重要作用,。Andreas Ladurner及其同事描述了FACT的Spt16M伴侶區(qū)域與H2A-H2B二聚物之間所形成的復(fù)合物的晶體結(jié)構(gòu)以及FACT的“異二聚”區(qū)域的結(jié)構(gòu)。Spt16M與組蛋白發(fā)生多種相互作用,，同時(shí)似乎也阻斷H2B與DNA的相互作用,，這有可能解釋FACT何以能使核小體失去穩(wěn)定性。（生物谷Bioon.com）
生物谷推薦英文摘要：
Nature doi:10.1038/nature12242
Structural basis of histone H2A–H2B recognition by the essential chaperone FACT
Maria Hondele,Tobias Stuwe, Markus HasslerFelix Halbac,Andrew Bowmn,Elisa T. Zhng,Bianca Nijmejer,Christiane Kothoff,VladimirRybin Stefan Amacher, Ed Hurt & Andreas G. Ladurner
Facilitates chromatin transcription (FACT) is a conserved histone chaperone that reorganizes nucleosomes and ensures chromatin integrity during DNA transcription, replication and repair. Key to the broad functions of FACT is its recognition of histones H2A–H2B (ref. 2). However, the structural basis for how histones H2A–H2B are recognized and how this integrates with the other functions of FACT, including the recognition of histones H3–H4 and other nuclear factors, is unknown. Here we reveal the crystal structure of the evolutionarily conserved FACT chaperone domain Spt16M from Chaetomium thermophilum, in complex with the H2A–H2B heterodimer. A novel ‘U-turn’ motif scaffolded onto a Rtt106-like module embraces the α1 helix of H2B. Biochemical and in vivo assays validate the structure and dissect the contribution of histone tails and H3–H4 towards Spt16M binding. Furthermore, we report the structure of the FACT heterodimerization domain that connects FACT to replicative polymerases. Our results show that Spt16M makes several interactions with histones, which we suggest allow the module to invade the nucleosome gradually and block the strongest interaction of H2B with DNA. FACT would thus enhance ‘nucleosome breathing’ by re-organizing the first 30 base pairs of nucleosomal histone–DNA contacts. Our snapshot of the engagement of the chaperone with H2A–H2B and the structures of all globular FACT domains enable the high-resolution analysis of the vital chaperoning functions of FACT, shedding light on how the complex promotes the activity of enzymes that require nucleosome reorganization.
