生物谷報(bào)道：美國(guó)Durham大學(xué)科學(xué)家Wilbanks等人發(fā)現(xiàn)ß-arrestins通過(guò)調(diào)控hedgehog信號(hào)通路從而調(diào)節(jié)了斑馬魚(yú)的發(fā)育,。眾所周知,，ß-arrestins是典型的七次跨膜蛋白，而以往并不知道它與hedgehog存在關(guān)聯(lián),。這一研究發(fā)現(xiàn),，使人類(lèi)對(duì)模式生物zerbfish的發(fā)育機(jī)制有更清晰的認(rèn)識(shí),。這篇文章發(fā)表在本期的Science上。
Fig. 1. Western blot analysis of zebrafish ß-arrestin 2 expression. Lysate (500 ng protein) from human embryonic kidney (HEK) 293T cells transiently transfected with either pCDNA3.1(+) vector alone (lane 1) or with zebrafish ß-arrestin 2 cDNA (lane 2). Lysate extracts from zebrafish embryos (48 hours after fertilization, two per lane) that were not injected (lane 3) or were injected with VA-MO (lane 4) or ßarr2-MO (lane 5) were probed with antibody to trout ß-arrestin 2, followed by a secondary horseradish peroxidase–conjugated antibody to rabbit, and then visualized by enhanced chemiluminesence. Equal loading of protein was verified by stripping and reprobing the blot with antibodies to actin. Densitometer quantification of three independent experiments reveals an average of 96 ± 3% reduction in ß-arrestin 2 expression with ßarr-MO.
Fig. 2. Phenotypes of ßarr2-MO embryos and Smoothened mutants hi2329b and hi1640 at 24 hours after fertilization. (A to E) Lateral views of whole zebrafish bodies. (F to J) Lateral views of somites, dorsal toward the top, anterior to the right. (K to O) S58 antibody staining of embryos. (P to T) 4D9 antibody staining of embryos.
Fig. 3. In situ hybridizations of zebrafish embryos at 27 hours after fertilization for downstream genes of the Hh signaling pathway. Embryos were not injected (NI) or were injected, as indicated, with MO to visual cone arrestin or with ß-arrestin 2 mRNA. (A to F) In situ hybridization with nkx2.2 probe. Shh mRNA (100 ng) was injected either alone (D), with VA-MO (E), or with ßarr2-MO (F). Shown are lateral views (dorsal toward the top, anterior to the left). (G to L) Double in situ hybridization with shh (blue stain, white arrows indicating expression at the midline) and ptc (red stain, dark arrows) probes on whole mounts [(G) to (I)] or sectioned embryos [(J) to (L)].
Fig. 4. Loss of slow muscle fiber development in ßarr2-MO is restored with DNPKA mRNA or Su(fu)-MO. Quantitative analysis of slow muscle fiber number per somite in embryos 24 hours after fertilization, injected as indicated. Embryos were stained with S58 antibody. Slow muscle fibers in three to five somites on 20 embryos per experiment were counted.
全文見(jiàn)：http://www.sciencemag.org/cgi/content/full/306/5705/2264
相關(guān)報(bào)道：
RNA干擾的果蠅Hedgehog途徑調(diào)控蛋白篩選
Sonic Hedgehog (SHH)受體ptc1調(diào)節(jié)細(xì)胞周期
Sonic Hedgehog (Shh) 信號(hào)
有利于細(xì)胞外信號(hào)分子Hedgehog分布的細(xì)胞膜上的通道
《Science》:肢體生長(zhǎng)的限制
肢體不對(duì)稱(chēng)發(fā)育中Hox基因扮演雙重角色
斑馬魚(yú)研究揭示胚胎血的形成機(jī)制
斑馬魚(yú)可引領(lǐng)科學(xué)家們尋找新療法
我國(guó)科學(xué)家發(fā)現(xiàn)調(diào)控轉(zhuǎn)化生長(zhǎng)因子的新機(jī)制
