《中國神經(jīng)再生研究（英文版）》雜志于2012年12月36期出版的一項(xiàng)關(guān)于“Biomechanical analysis of optic nerve injury treated by compound light granules and ciliary neurotrophic factor”的研究發(fā)現(xiàn),，①從生物力學(xué)和組織學(xué)角度觀察中藥復(fù)光顆粒和睫狀神經(jīng)營養(yǎng)因子對(duì)視神經(jīng)損傷兔的治療效果,。②以縱向拉伸的方法評(píng)估視神經(jīng)的應(yīng)力、應(yīng)變等數(shù)據(jù)，以回歸分析的方法建立視神經(jīng)應(yīng)力-應(yīng)變關(guān)系表達(dá)式,，進(jìn)行定量分析,。③視神經(jīng)損傷后其拉伸彈性限度應(yīng)變、彈性限度應(yīng)力,、最大應(yīng)力,、最大應(yīng)變均明顯減小，抗拉伸承載能力降低,。④中藥復(fù)光顆粒和睫狀神經(jīng)營養(yǎng)因子均可改善損傷視神經(jīng)的組織形態(tài),，提高視神經(jīng)拉伸力學(xué)相關(guān)指標(biāo)和抵抗載荷和變形的能力，二者聯(lián)合作用效果更好,。作者認(rèn)為,，復(fù)光顆粒和睫狀神經(jīng)營養(yǎng)因子均可從組織學(xué)和生物力學(xué)方面減輕視神經(jīng)損傷。(生物谷Bioon.com)

doi:10.3969/j.issn.1673-5374.2012.36.006
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Biomechanical analysis of optic nerve injury treated by compound light granules and ciliary neurotrophic factor

Yuying Jiang1, 2, Haitao Xu1, Jingxiang Liu2, Peng Li3, Yazhen Wu1

In this study, rabbit models of optic nerve injury were reproduced by the clamp method. After modeling, rabbit models were given one injection of 50 ng recombinant human ciliary neurotrophic factor into the vitreous body and/or intragastric injection of 4 g/kg compound light granules containing Radix Angelicae Sinensis and Raidix Paeoniae Alba at 4 days after modeling, once per day for 30 consecutive days. After administration, the animals were sacrificed and the intraorbital optic nerve was harvested. Hematoxylin-eosin staining revealed that the injured optic nerve was thinner and optic nerve fibers were irregular. After treatment with recombinant human ciliary neurotrophic factor, the arrangement of optic nerve fibers was disordered but they were not markedly thinner. After treatment with compound light granules, the arrangement of optic nerve fibers was slightly disordered and their structure was intact. After combined treatment with recombinant human ciliary neurotrophic factor and compound light granules, the arrangement of optic nerve fibers was slightly disordered and the degree of injury was less than after either treatment alone. Results of tensile mechanical testing of the optic nerve showed that the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly lower than the normal optic nerve. After treatment with recombinant human ciliary neurotrophic factor and/or compound light granules, the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly increased, especially after the combined treatment. These experimental findings indicate that compound light granules and ciliary neurotrophic factor can alleviate optic nerve injury at the histological and biochemical levels, and the combined treatment is more effective than either treatment alone.