一項(xiàng)關(guān)于“Secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell-line derived neurotrophic factor in co-culture of four cell types in cerebrospinal fluid-containing medium ”的研究顯示,，將人胚嗅鞘細(xì)胞,、人許旺細(xì)胞、人羊膜上皮細(xì)胞和人血管內(nèi)皮細(xì)胞在含腦脊液的完全培養(yǎng)基中聯(lián)合培養(yǎng),。結(jié)果證實(shí),，人胚嗅鞘細(xì)胞、人許旺細(xì)胞,、人羊膜上皮細(xì)胞和人血管內(nèi)皮細(xì)胞在含腦脊液培養(yǎng)基中聯(lián)合培養(yǎng)可促進(jìn)培養(yǎng)液中神經(jīng)生長因子,、腦源性神經(jīng)營養(yǎng)因子和膠質(zhì)源性神經(jīng)營養(yǎng)因子的表達(dá)。脊髓損傷后減少移植部位腦脊液的外滲對(duì)移植細(xì)胞生存及分泌神經(jīng)營養(yǎng)因子有益,。

相關(guān)研究成果發(fā)表在《中國神經(jīng)再生研究（英文版）》雜志于2012年12月36期出版的雜志上,。(生物谷Bioon.com)

doi:10.3969/j.issn.1673-5374.2012.36.008
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Secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell-line derived neurotrophic factor in co-culture of four cell types in cerebrospinal fluid-containing medium

Sanjiang Feng1, Minghua Zhuang1, Rui Wu2

The present study co-cultured human embryonic olfactory ensheathing cells, human Schwann cells, human amniotic epithelial cells and human vascular endothelial cells in complete culture mediumcontaining cerebrospinal fluid. Enzyme linked immunosorbent assay was used to detect nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor secretion in the supernatant of co-cultured cells. Results showed that the number of all cell types reached a peak at 7–10 days, and the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor peaked at 9 days. Levels of secreted nerve growth factor were four-fold higher than brain-derived neurotrophic factor, which was three-fold higher than glial cell line-derived neurotrophic factor. Increasing concentrations of cerebrospinal fluid (10%, 20% and 30%) in the growth medium caused a decrease of neurotrophic factor secretion. Results indicated co-culture of human embryonic olfactory ensheathing cells, human Schwann cells, human amniotic epithelial cells and human vascular endothelial cells improved the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor. The reduction of cerebrospinal fluid extravasation at the transplant site after spinal cord injury is beneficial for the survival and secretion of neurotrophic factors from transplanted cells.