《中國神經(jīng)再生研究（英文版）》雜志于2012年12月36期出版的一項(xiàng)關(guān)于“Protective effects of Lingguizhugan decoction on amyloid-β peptide 25-35-induced cell injury: anti-inflammatory effects”的研究,，觀察了苓桂術(shù)甘湯對阿爾茨海默病細(xì)胞模型的保護(hù)作用及抗炎相關(guān)的機(jī)制,。發(fā)現(xiàn)苓桂術(shù)甘湯可提高淀粉樣β蛋白25-35損傷的人神經(jīng)母細(xì)胞瘤細(xì)胞SH-SY5Y的活力,，同時可抑制活化的小膠質(zhì)細(xì)胞BV-2培養(yǎng)液上清中白細(xì)胞介素1β,、白細(xì)胞介素6,、腫瘤壞死因子α及一氧化氮的含量,。作者認(rèn)為，苓桂術(shù)甘湯可通過抑制小膠質(zhì)細(xì)胞活化后炎性因子的過表達(dá)保護(hù)淀粉樣β蛋白25-35損傷的SH-SY5Y細(xì)胞,。(生物谷Bioon.com)

doi:10.3969/j.issn.1673-5374.2012.36.003
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Protective effects of Lingguizhugan decoction on amyloid-beta peptide (25–35)-induced cell injury Anti-inflammatory effects

Feifei Xi1, Feng Sang2, Chunxiang Zhou1, 3, Yun Ling1

In the present study, a human neuroblastoma cell line (SH-SY5Y) and BV-2 microglia were treated with amyloid-β peptide (25–35), as a model of Alzheimer’s disease, to evaluate the protective effects of 10-3–10-8 g/mL Lingguizhugan decoction and to examine the underlying anti-inflammatory mechanism. Lingguizhugan decoction significantly enhanced the viability of SH-SY5Y cells with amyloid-β peptide-induced injury, and lowered levels of interleukin-1β, interleukin-6, tumor necrosis factor-α and nitric oxide in the culture supernatant of activated BV-2 microglia. The effects of 10-3 g/mL Lingguizhugan decoction were more significant. These results suggest that Lingguizhugan decoction can protect SH-SY5Y cells against amyloid-β peptide (25–35)-induced injury in a dose-dependent manner by inhibiting overexpression of inflammatory factors by activated microglia.