《中國神經(jīng)再生研究（英文版）》雜志于2012年12月35期出版的一項關于“Spinal cord biological safety of image-guided radiation therapy versus conventional radiation therapy”的研究，以犬T9-10椎體模擬腫瘤侵襲椎體，通過對脊髓組織病理學,、超微結構,、神經(jīng)元凋亡及凋亡相關蛋白Fas和熱休克蛋白70表達的檢測發(fā)現(xiàn)，脊柱圖像引導放療以對脊髓的可逆性損傷為主,，而普通放療以凋亡為主。在相同放射治療劑量下，與普通放療比較,，脊柱圖像引導放療引起的細胞凋亡數(shù)明顯降低，凋亡促進蛋白Fas的表達量明顯減少,，而凋亡抑制蛋白熱休克蛋白70表達量明顯增多,；同時脊髓細胞凋亡指數(shù)與Fas/熱休克蛋白70呈正相關。作者認為,，脊柱圖像引導放療對脊髓神經(jīng)元的生物安全性優(yōu)于普通放療,。(生物谷Bioon.com)

doi:10.3969/j.issn.1673-5374.2012.35.002
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Spinal cord biological safety of image-guided radiation therapy versus conventional radiation therapy

Wanlong Xu1, 2, Xilinbaoleri1, Hao Liu1, Ruozheng Wang1, Jingping Bai1

Tumor models were simulated in purebred Beagles at the T9-10 levels of the spinal cord and treated with spinal image-guided radiation therapy or conventional radiation therapy with 50 or 70 Gy total radiation. Three months after radiation, neuronal injury at the T9-10 levels was observed, including reversible injury induced by spinal image-guided radiation therapy and apoptosis induced by conventional radiation therapy. The number of apoptotic cells and expression of the proapoptotic protein Fas were significantly reduced, but expression of the anti-apoptotic protein heat shock protein 70 was significantly increased after image-guided radiation therapy compared with the conventional method of the same radiation dose. Moreover, the spinal cord cell apoptotic index positively correlated with the ratio of Fas/heat shock protein 70. These findings indicate that 3 months of radiation therapy can induce a late response in the spinal cord to radiation therapy; image-guided radiation therapy is safer and results in less neuronal injury compared with conventional radiation therapy.