Mycosystema
菌 物 學(xué) 報(bào) 15 March 2008, 27(2): 224-229
[email protected]
ISSN1672-6472 CN11-5180Q
2008 Institute of Microbiology, CAS, all rights reserved.
左登平 李春如 黃勃 何亞瓊 樊美珍 李增智
安徽農(nóng)業(yè)大學(xué)微生物防治省重點(diǎn)實(shí)驗(yàn)室 合肥 230036
摘 要：采用PCR技術(shù),，以rDNA-ITS區(qū)為分子指標(biāo),，對(duì)臺(tái)灣蟲(chóng)草Cordyceps formosana及其無(wú)性型黃山被毛孢Hirsutella huangshanensis進(jìn)行測(cè)序和比較分析,，以進(jìn)一步確定兩者的對(duì)應(yīng)關(guān)系。結(jié)果表明：兩者的序列完全相同,，從而在分子水平上證明了黃山被毛孢是臺(tái)灣蟲(chóng)草的無(wú)性階段,。系統(tǒng)進(jìn)化樹(shù)顯示臺(tái)灣蟲(chóng)草的無(wú)性型歸為被毛孢屬較為合理。
關(guān)鍵詞：黃山被毛孢,，rDNA-ITS 區(qū),，蟲(chóng)生真菌
Molecular identification for connection of Cordyceps formosana and its Hirsutella anamorph
ZUO Deng-Ping LI Chun-Ru* HUANG Bo HE Ya-Qiong FAN Mei-Zhen LI Zeng-Zhi
Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China
Abstract: The genes of the 5.8S rRNA and the complete internal transcribed spacer (ITS) regions from Cordyceps formosana and Hirsutella huangshanensis were sequenced and compared. The results showed that Cordyceps formosana and Hirsutella huangshanensis had the same ITS1-5.8S-ITS2 nucleotide sequences, strongly supporting the fact that Cordyceps formosana was the teleomorph of Hirsutella huangshanensis. Molecular phylogenetic analysis demonstrated that the conidial state of C. formosana belonged to the genus Hirsutella.
Key words: Hirsutela huangshanensis, rDNA-ITS, entomopathogenic fungi
