日本東京大學(xué)研究人員在3日出版的美國《細(xì)胞》雜志上發(fā)表論文說，他們成功利用大鼠的誘導(dǎo)多能干細(xì)胞（iPS細(xì)胞）培育出小鼠的胰腺,。
東京大學(xué)教授中內(nèi)啟光率領(lǐng)的研究小組首先通過基因手段,，培育出體內(nèi)缺少胰臟生成基因的雌性小鼠,，這種小鼠的后代出生后將沒有胰腺。隨后,，研究人員讓這些經(jīng)過基因改造的雌性小鼠交配,，并在其受精卵中注入來自正常大鼠皮膚的誘導(dǎo)多能干細(xì)胞,。結(jié)果,，出生的小鼠體內(nèi)擁有了能正常發(fā)揮作用的胰腺。
目前,，使用誘導(dǎo)多能干細(xì)胞開展的再生醫(yī)療研究主要集中在對臟器和組織的修復(fù)上,，雖然通過這種干細(xì)胞在體內(nèi)培育器官的研究尚處起步階段，但相關(guān)研究成果為再生醫(yī)療領(lǐng)域的研究帶來了新希望,。（生物谷Bioon.com）
生物谷推薦原文出處：
Cell doi：10.1016/j.cell.2010.07.039
Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells
Toshihiro Kobayashi, Tomoyuki Yamaguchi, Sanae Hamanaka, Megumi Kato-Itoh, Yuji Yamazaki, Makoto Ibata, Hideyuki Sato, Youn-Su Lee, Jo-ichi Usui, A.S. Knisely, Masumi Hirabayashi, Hiromitsu Nakauchi
The complexity of organogenesis hinders in vitro generation of organs derived from a patient's pluripotent stem cells (PSCs), an ultimate goal of regenerative medicine. Mouse wild-type PSCs injected into Pdx1/ (pancreatogenesis-disabled) mouse blastocysts developmentally compensated vacancy of the pancreatic developmental niche, generating almost entirely PSC-derived pancreas. To examine the potential for xenogenic approaches in blastocyst complementation, we injected mouse or rat PSCs into rat or mouse blastocysts, respectively, generating interspecific chimeras and thus confirming that PSCs can contribute to xenogenic development between mouse and rat. The development of these mouse/rat chimeras was primarily influenced by host blastocyst and/or foster mother, evident by body size and species-specific organogenesis. We further injected rat wild-type PSCs into Pdx1/ mouse blastocysts, generating normally functioning rat pancreas in Pdx1/ mice. These data constitute proof of principle for interspecific blastocyst complementation and for generation in vivo of organs derived from donor PSCs using a xenogenic environment.
