近期Nucleic Acids Research在線(xiàn)發(fā)表了上海生化與細(xì)胞所王恩多研究組的研究論文：人細(xì)胞質(zhì)亮氨酰-tRNA合成酶編校非對(duì)應(yīng)氨基酸的模塊式途徑,。
為了防止?jié)撛诘牡鞍踪|(zhì)錯(cuò)誤合成,，某些氨基酰-tRNA合成酶具有編校功能去除錯(cuò)誤活化的氨基酸或者錯(cuò)誤接載的氨基酰化tRNA,，通過(guò)控制蛋白質(zhì)的生物合成原料的質(zhì)量,，對(duì)蛋白質(zhì)合成進(jìn)行質(zhì)量控制,。該實(shí)驗(yàn)室已經(jīng)證明亮氨酰-tRNA合成酶(LeuRS)能夠通過(guò)不依賴(lài)-或依賴(lài)tRNA轉(zhuǎn)移前編校途徑水解誤活化的非對(duì)應(yīng)氨基酸,，即使誤活化的氨基酸轉(zhuǎn)移到tRNA分子上也可以通過(guò)轉(zhuǎn)移后編校途徑將其水解,。 LeuRS通過(guò)多條編校途徑在反應(yīng)的各個(gè)步驟清除錯(cuò)誤的反應(yīng)產(chǎn)物和中間物。
王恩多研究組的博士研究生陳鑫和馬晶晶成功地首次在大腸桿菌基因表達(dá)體系中得到了高活力的人胞質(zhì)亮氨酰-tRNA合成酶(hcLeuRS)和亮氨酸t(yī)RNA,，建立了一個(gè)高效的測(cè)定hcLeuRS合成和編?；盍Ψ椒ê腕w外研究體系,。發(fā)現(xiàn)hcLeuRS對(duì)不同的非對(duì)應(yīng)氨基酸的脅迫采取不同的編校策略對(duì)產(chǎn)物進(jìn)行質(zhì)量控制,，雖然亮氨酸的類(lèi)似物正纈氨酸(Nva)和2-氨基丁酸(ABA)都能被錯(cuò)誤地載入tRNA分子的3’端，但主要通過(guò)轉(zhuǎn)移后的編校途徑去除Nva,，去除ABA則偏愛(ài)轉(zhuǎn)移前的編校途徑,。轉(zhuǎn)移后編校作為tRNA氨基酰化最后的一個(gè)質(zhì)量控制的檢查點(diǎn)對(duì)于避免氨基酸的錯(cuò)誤摻入發(fā)揮著至關(guān)重要的作用,。該研究結(jié)果將有助于更加深入,、全面地認(rèn)識(shí)生物進(jìn)化過(guò)程中產(chǎn)生的模塊式編校途徑在保持遺傳信息由RNA傳遞到蛋白質(zhì)的精確性發(fā)揮著重要作用。
該研究得到中國(guó)科學(xué)院,，科技部重大科學(xué)研究計(jì)劃,，國(guó)家自然科學(xué)基金委，上海市科委的資助,。（生物谷Bioon.com）
生物谷推薦英文摘要：
Nucleic Acids Research, doi:10.1093/nar/gkq763
Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase
Xin Chen1, Jing-Jing Ma1, Min Tan1, Peng Yao1, Qing-Hua Hu1, Gilbert Eriani2 and En-Duo Wang1,*
1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China and 2Architecture et Réactivité de l’ARN, Institut de Biologie Moléculaire et Cellulaire du CNRS, Université de Strasbourg, 15 rue René Descartes 67084 Strasbourg, France
To prevent potential errors in protein synthesis, some aminoacyl-transfer RNA (tRNA) synthetases have evolved editing mechanisms to hydrolyze misactivated amino acids (pre-transfer editing) or misacylated tRNAs (post-transfer editing). Class Ia leucyl-tRNA synthetase (LeuRS) may misactivate various natural and non-protein amino acids and then mischarge tRNALeu. It is known that the fidelity of prokaryotic LeuRS depends on multiple editing pathways to clear the incorrect intermediates and products in the every step of aminoacylation reaction. Here, we obtained human cytoplasmic LeuRS (hcLeuRS) and tRNALeu (hctRNALeu) with high activity from Escherichia coli overproducing strains to study the synthetic and editing properties of the enzyme. We revealed that hcLeuRS could adjust its editing strategy against different non-cognate amino acids. HcLeuRS edits norvaline predominantly by post-transfer editing; however, it uses mainly pre-transfer editing to edit -amino butyrate, although both amino acids can be charged to tRNALeu. Post-transfer editing as a final checkpoint of the reaction was very important to prevent mis-incorporation in vitro. These results provide insight into the modular editing pathways created to prevent genetic code ambiguity by evolution.
