卵巢癌往往到晚期才被發(fā)現(xiàn),，而等到發(fā)現(xiàn)時,，癌細(xì)胞已經(jīng)轉(zhuǎn)移到其他器官，難以通過化療等方法治療,。美國最新研究發(fā)現(xiàn),，一種MicroRNA可以有效對抗卵巢癌，這一發(fā)現(xiàn)將使醫(yī)生有可能采用傳統(tǒng)的化療方法治療卵巢癌,。
美國佐治亞理工學(xué)院的研究人員在美國新一期《婦科腫瘤學(xué)》雜志上報告說,，他們在實(shí)驗(yàn)中利用具有調(diào)控功能的MiRNA－429，使卵巢癌細(xì)胞轉(zhuǎn)變成不能轉(zhuǎn)移的狀態(tài),，從而使癌細(xì)胞無法擴(kuò)散,。
負(fù)責(zé)這項(xiàng)研究的約翰·麥克唐納指出,，這一發(fā)現(xiàn)將使醫(yī)生有可能將病人的癌細(xì)胞生命周期調(diào)回到可以采用傳統(tǒng)的化療方法治療的階段。（生物谷Bioon.com）
生物谷推薦英文摘要：
Gynecologic Oncology doi:10.1016/j.ygyno.2010.12.339
Overexpression of miR-429 induces mesenchymal-to-epithelial transition (MET) in metastatic ovarian cancer cells
Jing Chena, Lijuan Wanga, Lilya V. Matyuninaa, Christopher G. Hilla and John F. McDonald, a,
a Integrated Cancer Research Center, School of Biology and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA 30332-0363, USA
Objective
Ovarian cancer (OC) is the most lethal of all gynecological malignancies primarily due to the sloughing-off of highly metastatic cells from primary tumors and their subsequent spread throughout the peritoneal cavity. Since the epithelial-to-mesenchymal transition (EMT) of OC cells located at the periphery of primary tumors is essential to this process, molecular interventions that can block EMT are of potential clinical significance. Members of the miR200 family of microRNAs have been implicated in EMT in other cancers. Our purpose was to determine if miR200 family microRNAs may be involved in EMT in OC and of potential therapeutic value in reducing OC metastasis.
Methods
Gene expression profiles of two OC cell lines with different metastatic potentials were monitored using qRT-PCR (quantitative reverse transcription polymerase chain reaction). The effect of over-expression of a miR-200 family microRNA (miR-429) in metastatic OC cells was monitored on molecular (qRT-PCR and microarray) and functional (morphology, migration, invasiveness and anchorage independence assays) levels.
Results
Molecular profiling of two OC cell lines with differing metastatic potentials identified significant differences in previously established epithelial and mesenchymal cell biomarkers including E-cadherin, ZEB1, ZEB2, miR-205 and miR-200 family microRNAs. Ectopic overexpression of miR-429, a member of the miR-200 family of microRNAs, in mesenchymal-like OC cells resulted in reversal of the mesenchymal phenotype (mesenchymal–epithelial transition, MET).
Conclusions
Our results indicate that miR-429 may not only be a useful biomarker of EMT in ovarian cancer, but also of potential therapeutic value in abating OC metastasis.
