近日，Cancer Sci雜志上刊登的一項(xiàng)研究中,，腫瘤工作者探討分析了Snail的臨床意義,，Snail是一種鋅指轉(zhuǎn)錄因子，存在于胃癌的發(fā)展過程中,。
為了闡明Snail表達(dá)與胃癌細(xì)胞腫瘤干細(xì)胞樣表型和分化狀態(tài)之間的關(guān)系,，研究人員采用免疫印跡、RT-PCR技術(shù),、實(shí)時(shí)定量PCR和流式細(xì)胞儀技術(shù),。
免疫組織化學(xué)染色和評價(jià)10例正常胃標(biāo)本以及103臨床癌癥患者Snail的表達(dá)特征，統(tǒng)計(jì)分析發(fā)現(xiàn)胃癌組織中Snail高表達(dá),，高表達(dá)Snail的胃癌患者生存預(yù)后較差,。
此外研究結(jié)果表明：胃癌細(xì)胞腫瘤干細(xì)胞樣表型的分化狀態(tài)與Snail的表達(dá)有關(guān)聯(lián)。Snail在胃癌組織中的表達(dá)水平與腫瘤細(xì)胞的分化,，腫瘤生長,，遠(yuǎn)處轉(zhuǎn)移情況和腫瘤分期呈正相關(guān)性。Snail表達(dá)高的胃癌患者整體存活率比Snail表達(dá)低的患者明顯降低,。多因素回歸分析顯示,，Snail表達(dá)是胃癌患者生存預(yù)后的獨(dú)立預(yù)測。
因此,，研究數(shù)據(jù)表明,，Snail表達(dá)可能是一個(gè)可靠的獨(dú)立的胃癌預(yù)后因素，臨床干預(yù)Snail表達(dá)潛在的治療胃癌患者策略,。（生物谷：Bioon.com)
doi:10.1111/j.1349-7006.2012.02295.x
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Snail is an independent prognostic predictor for progression and patient survival of gastric cancer.
He H, Chen W, Wang X, Wang C, Liu F, Shen Z, Xu J, Gu J, Sun Y.
The present study investigated the clinical significance of Snail, a zinc-finger transcription factor, in the development and progression of gastric cancer. To elucidate the relationship between Snail expression and dedifferentiation status with cancer stem cell phenotype in gastric cancer cells, we used western blot analysis, RT-PCR, quantitative real-time PCR and flow cytometry. Immunohistochemistry staining and evaluation of Snail expression in 10 human normal gastric samples versus 103 clinicopathologically characterized gastric cancer tissues followed by statistical analyses were applied to evaluate the prognostic value of Snail expression for progression and patient survival of gastric carcinomas. The results showed that functional Snail expression interlinks dedifferentiation status with cancer stem cell phenotype in gastric cancer cells. In addition, expression levels of Snail in gastric cancer tissues were significantly associated with tumor cell differentiation, local tumor growth, lymph node status, distant metastasis and tumor stage. The overall survival rate of gastric cancer patients with high Snail expression was significantly lower than for those patients with low Snail expression. Multivariate Cox regression analyses showed that Snail expression is an independent prognostic predictor for patient survival of gastric carcinomas. Thus, our data suggest that Snail expression could be a reliable independent prognostic factor to predict gastric carcinoma progression, which might open a new avenue for potential clinical intervention with functional Snail expression in gastric cancer patients.
