RNA聚合酶-II（Pol II）是在基因轉(zhuǎn)錄中起中心作用的酶,，在真核細(xì)胞中制造所有的信使RNA,。蛋白編碼基因的轉(zhuǎn)錄是由Pol II和包括TFIIB在內(nèi)的一般性轉(zhuǎn)錄因子所形成的一個(gè)復(fù)合物啟動(dòng)的,。
Kostrewa等人確定了Pol II/TFIIB復(fù)合物的完整晶體結(jié)構(gòu),。該結(jié)構(gòu)及互補(bǔ)功能數(shù)據(jù)表明,，轉(zhuǎn)錄的啟動(dòng)有一個(gè)由6個(gè)步驟構(gòu)成的機(jī)制,，包括當(dāng)轉(zhuǎn)錄開始點(diǎn)被定位后所觸發(fā)的向RNA伸長(zhǎng)的過(guò)渡,。（生物谷Bioon.com）
生物谷推薦原始出處：
Nature 462, 323-330 (19 November 2009) | doi:10.1038/nature08548
RNA polymerase II–TFIIB structure and mechanism of transcription initiationnear-final version
Dirk Kostrewa1,3, Mirijam E. Zeller2,3, Karim-Jean Armache1,3,4, Martin Seizl1, Kristin Leike1, Michael Thomm2 & Patrick Cramer1
1 Gene Center Munich and Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universit?t München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
2 Institut für Biochemie, Genetik und Mikrobiologie, Universit?t Regensburg, Universit?tsstrasse 31, 93053 Regensburg, Germany
3 These authors contributed equally to this work.
4 Present address: Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA.
5 Correspondence to: Patrick Cramer1 Correspondence and requests for materials should be addressed to P.C.
To initiate gene transcription, RNA polymerase II (Pol II) requires the transcription factor IIB (B). Here we present the crystal structure of the complete Pol II–B complex at 4.3 ? resolution, and complementary functional data. The results indicate the mechanism of transcription initiation, including the transition to RNA elongation. Promoter DNA is positioned over the Pol II active centre cleft with the 'B-core' domain that binds the wall at the end of the cleft. DNA is then opened with the help of the 'B-linker' that binds the Pol II rudder and clamp coiled-coil at the edge of the cleft. The DNA template strand slips into the cleft and is scanned for the transcription start site with the help of the 'B-reader' that approaches the active site. Synthesis of the RNA chain and rewinding of upstream DNA displace the B-reader and B-linker, respectively, to trigger B release and elongation complex formation.
