生物谷報道：最新由美國32個研究單位參與的一項大型研究,，以轉(zhuǎn)錄組水平為核心，全面揭示從基因組的表達(dá),，轉(zhuǎn)錄組水平，表型之間的相互關(guān)系,。
我們知道,，基因芯片目前常用于腫瘤等疾病的檢測,。然而,，最大的困難不是檢測本身,，而是芯片等高通量檢測后得到的大量數(shù)據(jù),，如何分析和認(rèn)識這些數(shù)據(jù)，從中發(fā)現(xiàn)最有價值的資料,，這是面對研究者的最大挑戰(zhàn),。本期Nature發(fā)表了由美國３２家研究單位共同參與的一項大型研究,，首次基因結(jié)構(gòu)性網(wǎng)絡(luò)知識全面從基因組水平分析人體的蛋白質(zhì),，小分子和表型與疾病之間的關(guān)系,。他們選擇了內(nèi)毒素炎癥作為模型,，研究了血液中的淋巴細(xì)胞對炎癥的反應(yīng),，全面揭示了淋巴細(xì)胞對急性系統(tǒng)性炎癥的反應(yīng),，包括淋巴細(xì)胞的生物代謝的紊亂,、基因轉(zhuǎn)錄地調(diào)節(jié)等,。這篇研究成果提供了全面揭示了淋巴細(xì)胞與內(nèi)在免疫系統(tǒng)的關(guān)系，為人類更深入地了解從基因表達(dá)到表型,，以及疾病之間的分子網(wǎng)絡(luò)機(jī)制提供依據(jù),。
A prototypical inflammatory cell was constructed from 292 representative genes involved in inflammation and innate immunity. Genes for which the expression statistically increased from baseline are coloured red, those for which expression decreased are shown in blue. a, Composite changes in apparent expression over 24 h, identifying nodes and interactions. b, Temporal changes in apparent expression. The response to endotoxin administration in blood leukocytes can be viewed as an integrated cell-wide response, propagating and resolving over time.
a, The network consists of 1,214 genes showing perturbed expression, and 342 genes highly interconnected to this group (red, increased; blue, decreased expression). b, Selected regions of the network, highlighting several groups of genes. Group 1, mitochondrial respiratory chain complex I (NDUF genes). Group 2, mitochondrial respiratory chain complex III (UQCR genes). Group 3, ATP synthase complex (ATP5 genes). Group 4, pyruvate dehydrogenase complex. Group 5, mitochondrial permeability transition pore complex. Group 6, elongation initiation factor complex (EIF3 genes). Group 7, ribosomal proteins (RPL, RPS genes). Group 8, COP9 signallosome (COPS genes). Group 9, proteasome (PSM genes).
原始來源：
Steve E. Calvano, Wenzhong Xiao, Daniel R. Richards, Ramon M. Felciano, Henry V. Baker, Raymond J. Cho, Richard O. Chen, Bernard H. Brownstein, J. Perren Cobb, S. Kevin Tschoeke, Carol Miller-Graziano, Lyle L. Moldawer, Michael N. Mindrinos, Ronald W. Davis, Ronald G. Tompkins, Stephen F. Lowry and Inflamm and Host Response to Injury Large Scale Collab. Res. Program. A network-based analysis of systemic inflammation in humans. Nature 437, 1032-1037
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