摘 要：對于蛋白質(zhì)組的研究離不開分析技術的支撐。由于樣品及其基質(zhì)的復雜性,，為了實現(xiàn)蛋白質(zhì)的高通量,、高靈敏度、快速分析鑒定,，必須發(fā)展與之匹配的新技術與新方法,。多維高效液相色譜/毛細管電泳技術,，部分彌補了傳統(tǒng)2D PAGE的不足，近年來,，在蛋白質(zhì)分離鑒定方面取得了最令人矚目的成績。本文分別從多維液相色譜分離技術,、多維毛細管電泳蛋白質(zhì)分離平臺,、微柱液相-毛細管電泳聯(lián)用技術、極端pH蛋白質(zhì)的分離分析和蛋白質(zhì)的在線富集技術等方面對蛋白質(zhì)組學研究中在新技術與新方法方面近期取得的成果加以系統(tǒng)闡述,。
關鍵詞：蛋白質(zhì)組,；多維分離；高效液相色譜,；毛細管電泳
New technology and method for proteomics research
ZHANG Weibing, ZHANG Lihua, ZHANG Yukui*
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Abstract: There is indispensable analysis technology for proteomics research. Because of the complexity of sample and matrix, for high throughput, high sensitivity and rapid identification in proteomics research, we need develop new technology and method to match this demand. Multidimensional HPLC/CE technology, partly supply the limitation of traditional 2D PAGE. Recently, they gained notable achievements in protein separation and identification. In this paper, we comprehensively elaborate the achievements from new technology and method for proteomics research as follows: Multi-dimensional HPLC; Multi-dimensional CE; mHPLC-CE; extreme PH protein analysis and on-line protein concentration technology.
Key words: proteomics; multi- dimension; HPLC; capillary electrophoresis
全文見附件1
附件1下載: 蛋白質(zhì)組研究中分離新技術與新方法.pdf
