使用全基因組寡核苷酸微陣列調(diào)查LYP9 水稻轉(zhuǎn)錄組譜及其親本，中科院遺傳所朱立煌,、農(nóng)科院以及國家雜交水稻工程技術(shù)研究中心的科學(xué)家研究了一種超級(jí)雜交水稻品種的遺傳背景和分子機(jī)制,。這種水稻由中國雜交水稻之父袁隆平在上世紀(jì)70年代開發(fā)，名為LYP9,，是解決中國糧食問題的水稻品種之一,。
聚類分析表明F1代雜交種表達(dá)譜匯集并超越了親本的優(yōu)勢，從22266個(gè)表達(dá)基因中發(fā)現(xiàn)了7078個(gè)基因均被7個(gè)樣本表達(dá),。經(jīng)過兩親本（DGPP）之間比較和雜交種與親本之間比較（DGHP）表明,，DGHP的能量代謝與轉(zhuǎn)運(yùn)基因表達(dá)要多于DGPP。這些差異表達(dá)基因,，尤其是參與碳水化合物代謝的基因,，可能是控制雜種優(yōu)勢的候選者。（生物谷Bioon.com）
生物谷推薦原始出處：
PNAS April 16, 2009, doi: 10.1073/pnas.0902340106
A transcriptomic analysis of superhybrid rice LYP9 and its parents
Gang Weia,b,1, Yong Taoa,b,1, Guozhen Liuc,d,1, Chen Chenc, Renyuan Luob, Hongai Xiaa, Qiang Gana,b, Haipan Zengc, Zhike Luc, Yuning Hanc, Xiaobing Lia, Guisheng Songa, Hongli Zhaia, Yonggang Penga, Dayong Lia, Honglin Xua, Xiaoli Weia, Mengliang Caoe, Huafeng Denge, Yeyun Xine, Xiqin Fue, Longping Yuane,2, Jun Yuc,2, Zhen Zhua,2 and Lihuang Zhua,2
aState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
bGraduate School of the Chinese Academy of Sciences, Beijing 100039, China;
cKey Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China;
dCollege of Life Sciences, Hebei Agricultural University, Baoding 071001, China; and
eNational Hybrid Rice Research and Development Center, Changsha 410125, China
By using a whole-genome oligonucleotide microarray, designed based on known and predicted indica rice genes, we investigated transcriptome profiles in developing leaves and panicles of superhybrid rice LYP9 and its parental cultivars 93-11 and PA64s. We detected 22,266 expressed genes out of 36,926 total genes set collectively from 7 tissues, including leaves at seedling and tillering stages, flag leaves at booting, heading, flowering, and filling stages, and panicles at filling stage. Clustering results showed that the F1 hybrid's expression profiles resembled those of its parental lines more than that which lies between the 2 parental lines. Out of the total gene set, 7,078 genes are shared by all sampled tissues and 3,926 genes (10.6% of the total gene set) are differentially expressed genes (DG). As we divided DG into those between the parents (DGPP) and between the hybrid and its parents (DGHP), the comparative results showed that genes in the categories of energy metabolism and transport are enriched in DGHP rather than in DGPP. In addition, we correlated the concurrence of DG and yield-related quantitative trait loci, providing a potential group of heterosis-related genes.
