由中國國家玉米改良中心,、威斯康星大學(xué)以及中國農(nóng)業(yè)部聯(lián)合組成的研究團(tuán)隊已繪制完成黃瓜基因組的綜合遺傳學(xué)和細(xì)胞遺傳學(xué)圖譜,，這對于育種家培育更好的葫蘆科作物而言是十分重要的資源,。該圖譜包括分布在7個連鎖群的995個簡單重復(fù)序列（SSR）標(biāo)記,，總長為573厘摩,。這995個SSRs中,，有49%,、26%和22%是分別屬于甜瓜、西瓜和南瓜,。應(yīng)用FISH分析已確定了各連鎖群間和黃瓜染色體組的關(guān)系,。
這一圖譜是首個報道的葫蘆科植物的基因組圖譜。Yi Ren及其同事在已發(fā)表在PloS ONE的一篇論文中指出,，這一圖譜的有效性將推動整個基因組測序和定位克隆技術(shù)的發(fā)展,，加強(qiáng)輔助標(biāo)記選擇，并為研究葫蘆科植物的共性提供更佳選擇,。（生物谷Bioon.com）
生物谷推薦原始出處：
PLoS ONE 4(6): e5795. doi:10.1371/journal.pone.0005795
An Integrated Genetic and Cytogenetic Map of the Cucumber Genome
Yi Ren1#, Zhonghua Zhang1#, Jinhua Liu2#, Jack E. Staub3, Yonghua Han2, Zhouchao Cheng1, Xuefeng Li1, Jingyuan Lu2, Han Miao1, Houxiang Kang1, Bingyan Xie1, Xingfang Gu1, Xiaowu Wang1, Yongchen Du1, Weiwei Jin2*, Sanwen Huang1*
1 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Sino-Dutch Joint Lab of Horticultural Genomics, Opening Lab of Genetic Improvement of Agricultural Crops of Ministry of Agriculture, Beijing, China, 2 National Maize Improvement Center of China, Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China, 3 USDA, ARS, Vegetable Crops Research Unit, Department of Horticulture, University of Wisconsin, Madison, Wisconsin, United States of America
The Cucurbitaceae includes important crops such as cucumber, melon, watermelon, squash and pumpkin. However, few genetic and genomic resources are available for plant improvement. Some cucurbit species such as cucumber have a narrow genetic base, which impedes construction of saturated molecular linkage maps. We report herein the development of highly polymorphic simple sequence repeat (SSR) markers originated from whole genome shotgun sequencing and the subsequent construction of a high-density genetic linkage map. This map includes 995 SSRs in seven linkage groups which spans in total 573 cM, and defines ~680 recombination breakpoints with an average of 0.58 cM between two markers. These linkage groups were then assigned to seven corresponding chromosomes using fluorescent in situ hybridization (FISH). FISH assays also revealed a chromosomal inversion between Cucumis subspecies [C. sativus var. sativus L. and var. hardwickii (R.) Alef], which resulted in marker clustering on the genetic map. A quarter of the mapped markers showed relatively high polymorphism levels among 11 inbred lines of cucumber. Among the 995 markers, 49%, 26% and 22% were conserved in melon, watermelon and pumpkin, respectively. This map will facilitate whole genome sequencing, positional cloning, and molecular breeding in cucumber, and enable the integration of knowledge of gene and trait in cucurbits.
