北京生命科學(xué)研究所鄧興旺實(shí)驗(yàn)室在Plant Molecular Biology雜志上在線發(fā)表題為 “ Genome-wide profiling of histone H3 lysine 9 acetylation and dimethylation in Arabidopsis reveals correlation between multiple histone marks and gene expression”的文章,。 該文章通過(guò)ChIP-chip技術(shù), 對(duì)擬南芥基因組水平組蛋白修飾與基因表達(dá)之間的關(guān)系進(jìn)行了研究,。
組蛋白H3的賴氨酸9位點(diǎn)可以被乙酰化及單,、二或三甲基化,，這些組蛋白的修飾狀態(tài)對(duì)基因的表達(dá)以及染色質(zhì)的組織結(jié)構(gòu)有一定的影響。在擬南芥中,，H3K9ac幾乎毫無(wú)例外地與轉(zhuǎn)錄激活相關(guān),，而H3K9me2則主要位于組成型異染色質(zhì)區(qū),。本文報(bào)道了擬南芥全基因組組蛋白H3K9ac和H3K9me2修飾的圖譜,，結(jié)果表明H3K9ac修飾5206個(gè)非TE編碼基因和321 TE編碼基因，而H3K9me2修飾2281個(gè)TE基因和1112個(gè)非TE編碼基因,。H3K9ac修飾集中在基因的5′末端,，在ATG位置有一個(gè)高峰，而H3K9me2修飾則覆蓋基因的整個(gè)編碼區(qū),。 H3K9ac修飾的基因的表達(dá)活性相對(duì)較高,，而H3K9me2則多分布在表達(dá)活性較低的基因。對(duì)本文中H3K9ac和H3K9me2的全基因組修飾的圖譜與已經(jīng)報(bào)道的H3K27me3和DNA甲基化修飾圖譜一起研究發(fā)現(xiàn),，同一基因上的組蛋白多個(gè)位點(diǎn)修飾和基因的表達(dá)活性之間存在相關(guān)性,。（生物谷Bioon.com）
更多應(yīng)用ChIP-chip技術(shù)研究：
Genome Research：推測(cè)組蛋白修飾與基因表達(dá)關(guān)系
Nature：組蛋白修飾對(duì)細(xì)胞分化控制機(jī)制
Cell：發(fā)現(xiàn)乳腺癌新型藥物靶標(biāo)
Biochem Biophys Res Commun：由BMP2誘導(dǎo)的Sox9基因表觀遺傳學(xué)研究
Nature：基因增強(qiáng)子的時(shí)空分布
中國(guó)首個(gè)ChIP技術(shù)論壇：ChIP Forum
生物谷推薦原始出處：
Plant Molecular Biology DOI:10.1007/s11103-009-9594-7
Genome-wide profiling of histone H3 lysine 9 acetylation and dimethylation in Arabidopsis reveals correlation between multiple histone marks and gene expression
Junli Zhou1, 2 , Xiangfeng Wang1, 2 , Kun He2, 3 , Jean-Benoit F. Charron2 , Axel A. Elling2 and Xing Wang Deng1, 2, 3
(1) National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, 102206 Beijing, People’s Republic of China
(2) Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
(3) Peking-Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, College of Life Sciences, Peking University, 100871 Beijing, People’s Republic of China
Lysine residue 9 of histone H3 can either be acetylated or mono-, di-, or tri-methylated. These epigenetic states have a diverse impact on regulating gene transcriptional activity and chromatin organization. H3K9ac is invariably correlated with transcriptional activation, whereas H3K9me2 has been reported to be mainly located in constitutive heterochromatin in Arabidopsis. Here, we present epigenetic landscapes for histone H3 lysine 9 acetylation (H3K9ac) and dimethylation (H3K9me2) in Arabidopsis seedlings. The results show that H3K9ac targeted 5,206 non-transposable element (non-TE) genes and 321 transposable elements (TEs), whereas H3K9me2 targeted 2,281 TEs and 1,112 non-TE genes. H3K9ac was biased towards the 5′ end of genes and peaked at the ATG position, while H3K9me2 tended to span the entire gene body. H3K9ac correlated with high gene expression, while H3K9me2 correlated with low expression. Analyses of H3K9ac and H3K9me2 with the available datasets of H3K27me3 and DNA methylation revealed a correlation between the occurrence of multiple epigenetic modifications and gene expression. Genes with H3K9ac alone were actively transcribed, while genes that were also modified by either H3K27me3 or DNA methylation showed a lower expression level, suggesting that a combination of repressive marks weakened the positive regulatory effect of H3K9ac. Furthermore, we observed a significant increase of the H3K9ac modification level of selected target genes in hda19 (histone deacetylase 19) mutant seedlings, which indicated that HDA19 plays an important role in regulating the level of H3K9ac and thereby influencing the transcriptional activity in young seedlings.
Electronic supplementary material The online version of this article (doi:10.1007/s11103-009-9594-7) contains supplementary material, which is available to authorized users.
