最新一期《實(shí)驗(yàn)動物學(xué)雜志·生態(tài)遺傳學(xué)和生理學(xué)》(4月號)在封面文章位置發(fā)表了中科院水生所關(guān)于“石斑魚性反轉(zhuǎn)和配子發(fā)生期間Sox3基因差異表達(dá)和動態(tài)變化”論文,。這篇論文是該所桂建芳研究員指導(dǎo)的博士研究生姚波和副研究員周莉等共同完成的，封面圖片顯示了SOX3在原始生殖細(xì)胞和初級卵母細(xì)胞中清晰的熒光定位圖像。
石斑魚是我國南方沿海地區(qū)網(wǎng)箱養(yǎng)殖的重要經(jīng)濟(jì)魚類之一。其在生活史中具有由雌變雄的性反轉(zhuǎn)過程，因此雄性親魚短缺是限制產(chǎn)業(yè)發(fā)展的主要因素之一,，同時它也是研究魚類生殖調(diào)控和性別分化分子機(jī)制的一個獨(dú)特對象。在國家“973”和“863”等計(jì)劃項(xiàng)目的支持下，從“十五”開始,，水生所魚類發(fā)育遺傳學(xué)和細(xì)胞工程學(xué)科組以南方沿海普遍養(yǎng)殖的斜帶石斑魚(E. Coioides)為研究對象，圍繞生殖內(nèi)分泌調(diào)控軸,，構(gòu)建了石斑魚下丘腦,、垂體和性腺的一系列cDNA文庫,，篩選和克隆到了一批參與生殖調(diào)控和性腺分化的重要功能基因，分析了一批基因隨著性反轉(zhuǎn)進(jìn)程在垂體,、性腺中或在胚胎發(fā)育過程中的時空表達(dá)圖譜,。剛剛在《實(shí)驗(yàn)動物學(xué)雜志·生態(tài)遺傳學(xué)和生理學(xué)》上發(fā)表的是這一系列研究的論文之一。
該文詳細(xì)報(bào)道了石斑魚性反轉(zhuǎn)和配子發(fā)生期間SOX3蛋白在不同發(fā)育階段生殖細(xì)胞中的定位及其動態(tài)變化,。在雌性性腺中,，SOX3蛋白存在于分化中的原始生殖細(xì)胞、卵原細(xì)胞和不同發(fā)育階段的卵母細(xì)胞核中,，而在雄性性腺中則存在于分化中的原始生殖細(xì)胞和支持細(xì)胞中,。當(dāng)SOX3持續(xù)表達(dá)時，SOX3陽性的原始生殖細(xì)胞朝卵原細(xì)胞和卵母細(xì)胞方向發(fā)育,；當(dāng)Sox3表達(dá)停止時,，原始生殖細(xì)胞則進(jìn)入精子發(fā)生過程。該文重要的創(chuàng)新點(diǎn)之一是巧妙地利用雌雄同體的石斑魚同時具有不同發(fā)育階段的雌性配子細(xì)胞和雄性配子細(xì)胞的特性,，通過一系列的免疫熒光定位觀察,，揭示Sox3的表達(dá)是原始生殖細(xì)胞進(jìn)入配子發(fā)生的一個時間檢驗(yàn)點(diǎn)，其表達(dá)是否持續(xù),，將決定配子發(fā)生向兩種截然不同的方向進(jìn)行,。因而這一發(fā)現(xiàn)證實(shí)了SOX3在卵子發(fā)生和配子細(xì)胞分化中的潛在調(diào)控作用，并確信SOX3在卵子發(fā)生中的作用比在精子發(fā)生中的作用要大得多,。
部分英文原文:
Research Article
Differential expression and dynamic changes of SOX3 during gametogenesis and sex reversal in protogynous hermaphroditic fish
Bo Yao, Li Zhou, Yang Wang, Wei Xia, Jian-Fang Gui *
State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan Center for Developmental Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan, China
email: Jian-Fang Gui ([email protected])
*Correspondence to Jian-Fang Gui, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Bo Yao and Li Zhou have contributed equally to this work.
Bo Yao and Li Zhou have contributed equally to this work.
Funded by:
The National Basic Research Program of China; Grant Number: 2004CB117401
The Innovation group project of Hubei Province; Grant Number: 2004ABC005
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Abstract
SOX3 has been suggested to play significant roles in gametogenesis and gonad differentiation of vertebrates, but the exact cellular localization evidence is insufficient and controversial. In this study, a protogynous hermaphrodite fish Epinephelus coioides is selected to analyze EcSox3 differential expression and the expression pattern in both processes of oogenesis and spermatogenesis by utilizing the advantages that gonad development undergoes transition from ovary to intersexual gonad and then to testis, and primordial germ cells and different stage cells during oogenesis and spermatogenesis are synchronously observed in the transitional gonads. The detailed and clear immunofluoresence localization indicates that significantly differential expression and dynamic changes of Sox3 occur in the progresses of gametogenesis and sex reversal, and EcSOX3 protein exists in the differentiating primordial germ cells, oogonia, and different stage oocytes of ovaries, and also in the differentiating primordial germ cells and the Sertoli cells of testis. One important finding is that the EcSox3 expression is a significant time point for enterable gametogenesis of primordial germ cells because EcSOX3 is obviously expressed and localized in primordial germ cells. As EcSox3 continues to express, the EcSOX3-positive primordial germ cells develop toward oogonia and then oocytes, whereas when EcSox3 expression is ceased, the EcSOX3-positive primordial germ cells develop toward spermatogonia. Therefore, the current finding of EcSOX3 in the differentiating primordial germ cells again confirms the potential regulatory role in oogenesis and germ cell differentiation. The data further suggest that SOX3, as a transcription factor, might have more important roles in oogenesis than in spermatogenesis. J. Exp. Zool. 307A:207-219, 2007. © 2007 Wiley-Liss, Inc.
