生物谷報道：國際頂尖植物學(xué)權(quán)威雜志“The Plant Cell”日前發(fā)表了武漢大學(xué)生命科學(xué)學(xué)院博士生陳榮智的研究論文,，該項研究成果對于雜交水稻的三系育種具有重要作用。
該論文題為《水稻尿苷二磷酸葡萄糖焦磷酸化酶基因Ugp1對花粉發(fā)育過程中的胼胝質(zhì)沉積是必需的,，它的共抑制導(dǎo)致一種新型溫敏核不育表型》,，運用電噴霧串聯(lián)質(zhì)譜鑒定出水稻差異表達(dá)蛋白UGPase并克隆了其編碼基因Ugp1，隨后應(yīng)用基因超量表達(dá)和RNA干擾技術(shù)通過水稻轉(zhuǎn)基因?qū)gp1基因的功能進(jìn)行了詳細(xì)研究,，闡明了Ugp1基因在水稻生長發(fā)育中的重要功能。同時還發(fā)現(xiàn)Ugp1共抑制水稻表現(xiàn)為一種新型的溫度敏感細(xì)胞核不育類型,，并證明Ugp1基因的溫度敏感型剪接是溫敏核不育Ugp1共抑制水稻育性恢復(fù)的分子基礎(chǔ),。
陳榮智是武漢大學(xué)生命科學(xué)學(xué)院2002級博士生，師從著名的植物遺傳學(xué)家何光存教授,。
原始出處:
First published online March 30, 2007; 10.1105/tpc.106.044123
The Plant Cell 19:847-861 (2007)
Rice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male Sterility[W],[OA]
Rongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He and Guangcun He1
Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
1 To whom correspondence should be addressed. E-mail [email protected] ; fax 86-27-68752327.
UDP-glucose pyrophosphorylase (UGPase) catalyzes the reversible production of glucose-1-phosphate and UTP to UDP-glucose and pyrophosphate. The rice (Oryza sativa) genome contains two homologous UGPase genes, Ugp1 and Ugp2. We report a functional characterization of rice Ugp1, which is expressed throughout the plant, with highest expression in florets, especially in pollen during anther development. Ugp1 silencing by RNA interference or cosuppression results in male sterility. Expressing a double-stranded RNA interference construct in Ugp1-RI plants resulted in complete suppression of both Ugp1 and Ugp2, together with various pleiotropic developmental abnormalities, suggesting that UGPase plays critical roles in plant growth and development. More importantly, Ugp1-cosuppressing plants contained unprocessed intron-containing primary transcripts derived from transcription of the overexpression construct. These aberrant transcripts undergo temperature-sensitive splicing in florets, leading to a novel thermosensitive genic male sterility. Pollen mother cells (PMCs) of Ugp1-silenced plants appeared normal before meiosis, but during meiosis, normal callose deposition was disrupted. Consequently, the PMCs began to degenerate at the early meiosis stage, eventually resulting in complete pollen collapse. In addition, the degeneration of the tapetum and middle layer was inhibited. These results demonstrate that rice Ugp1 is required for callose deposition during PMC meiosis and bridges the apoplastic unloading pathway and pollen development.
