2011年4月，Marine Biotechnology刊登了中科院水生生物研究所魚類基因工程學科組與北京大學生命科學院合作的封面研究論文：Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio),。
該文報道了轉透明顫菌血紅蛋白 (VHb) 提高斑馬魚的低氧耐受能力的研究,。采用顯微操作技術將VHb基因轉移到斑馬魚受精卵獲得轉基因斑馬魚,，經(jīng)過144-156小時的低氧(0.91 mg/l)脅迫后，發(fā)現(xiàn)轉VHb基因斑馬魚的生存率顯著高于對照魚,。該文同時研究了轉VHb基因魚耐受低氧脅迫的機制,，表明通過轉VHb基因有望研制具有低氧耐受能力的養(yǎng)殖魚類新品種,。
溶氧是影響水產(chǎn)養(yǎng)殖最重要的環(huán)境因子之一。溫度,、晝夜節(jié)律和季節(jié)變化以及富營養(yǎng)化作用等都會導致水體出現(xiàn)低氧或缺氧,，從而影響魚類生長、內(nèi)分泌調(diào)節(jié)和繁殖特性等,，甚至導致魚類死亡,。研制低氧耐受的養(yǎng)殖魚類新品種具有重要應用價值。
該論文是在水生所胡煒研究員和北京大學林忠平教授指導下,，由博士研究生管波和麻洪等人共同完成,。該研究得到了國家863和自然科學基金等項目的資助。封面圖片為eGFP標記的轉VHb基因斑馬魚胚胎及對照斑馬魚胚胎,。（生物谷Bioon.com）
生物谷推薦原文出處：
Marine Biotechnology Volume 13, Number 2, 336-344, DOI: 10.1007/s10126-010-9305-z
Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)
Bo Guan, Hong Ma, Yaping Wang, Yuanlei Hu, Zhongping Lin, Zuoyan Zhu and Wei Hu
Abstract
Aquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp β-actin promoter to create stable transgenic zebrafish. The survival rate of the 7-day-old F2 transgenic fish was significantly greater than that of the sibling controls under 2.5% O2 (dissolved oxygen (DO), 0.91 mg/l). Meanwhile, we investigated the relative expression levels of several marker genes (hypoxia-inducible factor alpha 1, heat shock cognate 70-kDa protein, erythropoietin, beta and alpha globin genes, lactate dehydrogenase, catalase, superoxide dismutase, and glutathione peroxidase) of transgenic fish and siblings after hypoxia exposure for 156 h. The expression profiles of the vhb transgenic zebrafish revealed that VHb could partially alleviate the hypoxia stress response to improve the survival rate of the fish. These results suggest that that vhb gene may be an efficient candidate for genetically modifying hypoxia tolerance in fish.
Keywords Vitreoscilla hemoglobin (VHb) – Hypoxia – Zebrafish (Danio rerio) – Transgenic fish
雜志封面為eGFP標記的轉VHb基因斑馬魚胚胎及對照斑馬魚胚胎
