生物谷報(bào)道：最近，中科院上海生命科學(xué)研究院神經(jīng)研究所袁小兵研究組的陳罡,、司馬健和金明等的研究首次證明了一種梯度表達(dá)的分泌性導(dǎo)向分子Semaphorin-3A指引了皮層神經(jīng)元的放射狀遷移,。采用在體子宮內(nèi)基因電轉(zhuǎn)技術(shù)，他們可長時(shí)程觀察一群皮層神經(jīng)元在體內(nèi)的遷移,。發(fā)現(xiàn)體內(nèi)通過阻斷Semaphorin-3A的信號可以導(dǎo)致遷移的停滯并伴隨神經(jīng)元伸展方向的錯(cuò)亂,。1月10日，Nature Neuroscience發(fā)表了該成果,。
體外實(shí)驗(yàn)也發(fā)現(xiàn)內(nèi)源性Semaphorin-3A的濃度梯度對于新生神經(jīng)元的正確遷移是必須的,，并且起吸引性導(dǎo)向作用。這些研究首次證明了神經(jīng)元的放射狀遷移受胞外導(dǎo)向分子的指引。這種遷移的趨化性導(dǎo)向假說有助于我們更好的理解高度有序的皮層結(jié)構(gòu)是如何形成的,。
該項(xiàng)研究工作得到了中國科學(xué)院,、“973”計(jì)劃、重要科學(xué)研究計(jì)劃,、國家自然科學(xué)基金委員會杰出青年基金及上海市啟明星計(jì)劃的資助,。（援引上海生命科學(xué)研究院）
生物谷推薦原始出處：
Nature Neuroscience 11, 36 - 44 (2007)
Published online: 2 December 2007 | Corrected online: 9 December 2007 | doi:10.1038/nn2018
Semaphorin-3A guides radial migration of cortical neurons during development
Gang Chen1,2,3, Jian Sima1,2,3, Ming Jin1,3, Kai-yu Wang1,2, Xiao-jing Xue1,2, Wang Zheng1,2, Yu-qiang Ding1 & Xiao-bing Yuan1
Abstract
Postmitotic neurons in the developing cortex migrate along radial glial fibers to their proper location in the cortical plate and form the layered structure. Here we report that the radial migration of rat layer II/III cortical neurons requires guidance by the extracellular diffusible factor Semaphorin-3A (Sema3A). This factor is expressed in a descending gradient across the cortical layers, whereas its receptor neuropilin-1 (NP1) is highly expressed in migrating neurons. Downregulation or conditional knockout of NP1 in newborn cortical neurons impedes their radial migration by disrupting their radial orientation during migration without altering their cell fate. Studies in cultured cortical slices further show that the endogenous gradient of Sema3A is required for the proper migration of newborn neurons. In addition, transwell chemotaxis assays show that isolated newborn neurons are attracted by Sema3A. Thus, Sema3A may function as a chemoattractive guidance signal for the radial migration of newborn cortical neurons toward upper layers.
Institute of Neuroscience and Key State Laboratory of Neurobiology, Shanghai Institute for Biological Sciences, 320 Yue Yang Road, Shanghai 200031, China.
Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China.
These authors contributed equally to this work.
Correspondence to: Xiao-bing Yuan1 e-mail: [email protected]
* In the version of the supplementary information originally published online for this manuscript, the legends for Supplementary Video #3 and Supplementary Video #4 were inadvertently switched. This error has now been corrected and the legends correctly describe the appropriate videos
