神經(jīng)干細(xì)胞移植治療脊髓損傷被證明是一種具有廣闊前景的治療方法,，但是神經(jīng)干細(xì)胞移植過程中由于缺少微環(huán)境的支持,，移植后細(xì)胞存活率低，影響移植效果,。膠原蛋白是天然的細(xì)胞外基質(zhì),，具有良好的細(xì)胞黏附性、低免疫原性,、可降解性,、被廣泛用于組織工程研究中,。安徽醫(yī)科大學(xué)沈強(qiáng)教授在《中國神經(jīng)再生研究（英文版）》雜志2013年2月第4期發(fā)表的一項(xiàng)關(guān)于“Growth and differentiation of neural stem cells in a three-dimensional collagen gel scaffold”的研究，利用Ⅰ型膠原蛋白形成的膠原凝膠三維支架作為載體培養(yǎng)神經(jīng)干細(xì)胞,，觀察神經(jīng)干細(xì)胞在膠原凝膠中生長時(shí)細(xì)胞特性的改變,。結(jié)果證實(shí)，膠原凝膠三維支架可以促進(jìn)神經(jīng)干細(xì)胞的增殖,、分化和突起的生長,，效果優(yōu)于懸浮培養(yǎng)。說明膠原凝膠是一種良好的生物支架材料,，有利于維持神經(jīng)干細(xì)胞的特性,，并能提高神經(jīng)干細(xì)胞向神經(jīng)元分化的比例。(生物谷Bioon.com)
DOI: 10.3969/j.issn.1673-5374.2013.04.003
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Growth and differentiation of neural stem cells in a three-dimensional collagen gel scaffold
Fei Huang1, Qiang Shen2, Jitong Zhao2
Collagen protein is an ideal scaffold material for the transplantation of neural stem cells. In this study, rat neural stem cells were seeded into a three-dimensional collagen gel scaffold, with suspension cultured neural stem cells being used as a control group. Neural stem cells, which were cultured in medium containing epidermal growth factor and basic fibroblast growth factor, actively expanded and formed neurospheres in both culture groups. In serum-free medium conditions, the processes extended from neurospheres in the collagen gel group were much longer than those in the suspension culture group. Immunofluorescence staining showed that neurospheres cultured in collagen gels were stained positive for nestin and differentiated cells were stained positive for the neuronal marker βIII-tubulin, the astrocytic marker glial fibrillary acidic protein and the oligodendrocytic marker 2’,3’-cyclic nucleotide 3'-phosphodiesterase. Compared with neurospheres cultured in suspension, the differentiation potential of neural stem cells cultured in collagen gels increased, with the formation of neurons at an early stage. Our results show that the three-dimensional collagen gel culture system is superior to suspension culture in the proliferation, differentiation and process outgrowth of neural stem cells.
