一項(xiàng)關(guān)于“Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells”的研究,，對(duì)小鼠孤雌胚胎干細(xì)胞和胚胎干細(xì)胞向神經(jīng)細(xì)胞分化的潛力進(jìn)行了比較。結(jié)果發(fā)現(xiàn)：（1）孤雌胚胎干細(xì)胞和胚胎干細(xì)胞的核型保持正常,，性染色體為XX,。（2）孤雌胚胎干細(xì)胞和受精胚來(lái)源的胚胎干細(xì)胞高表達(dá)多能性蛋白Oct4 mRNA及蛋白,，說(shuō)明細(xì)胞具有多向分化潛能。（3）經(jīng)誘導(dǎo)的孤雌胚胎干細(xì)胞和胚胎干細(xì)胞均表達(dá)神經(jīng)細(xì)胞標(biāo)志物Nestin,、β-Ⅲ微管蛋白及髓鞘堿性蛋白和神經(jīng)發(fā)生相關(guān)基因Sox-1, Nestin, GABA, Pax6, Zic5, Pitx1,，而多能性基因Oct4的表達(dá)顯著降低。（4）孤雌胚胎干細(xì)胞中Nestin和Pax6基因的表達(dá)明顯高于胚胎干細(xì)胞,。作者認(rèn)為,，孤雌胚胎干細(xì)胞比胚胎干細(xì)胞具有更強(qiáng)的向神經(jīng)細(xì)胞分化的潛力。此項(xiàng)研究結(jié)果發(fā)表在《中國(guó)神經(jīng)再生研究（英文版）》雜志2013年2月第4期上,。(生物谷Bioon.com)
DOI: 10.3969/j.issn.1673-5374.2013.04.001
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Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells
Xingrong Yan1, Yanhong Yang2, Wei Liu1, Wenxin Geng1, Huichong Du1, Jihong Cui1, Xin Xie1, Jinlian Hua3, Shumin Yu4, Liwen Li1, Fulin Chen1
Parthenogenetic embryonic stem cells have pluripotent differentiation potentials, akin to fertilized embryo-derived embryonic stem cells. The aim of this study was to compare the neuronal differentiation potential of parthenogenetic and fertilized embryo-derived embryonic stem cells. Before differentiation, karyotype analysis was performed, with normal karyotypes detected in both parthenogenetic and fertilized embryo-derived embryonic stem cells. Sex chromosomes were identified as XX. Immunocytochemistry and quantitative real-time PCR detected high expression of the pluripotent gene, Oct4, at both the mRNA and protein levels, indicating pluripotent differentiation potential of the two embryonic stem cell subtypes. Embryonic stem cells were induced with retinoic acid to form embryoid bodies, and then dispersed into single cells. Single cells were differentiated in N2 differentiation medium for 9 days. Immunocytochemistry showed parthenogenetic and fertilized embryo-derived embryonic stem cells both express the neuronal cell markers nestin, βIII-tubulin and myelin basic protein. Quantitative real-time PCR found expression of neurogenesis related genes (Sox-1, Nestin, GABA, Pax6, Zic5 and Pitx1) in both types of embryonic stem cells, and Oct4 expression was significantly decreased. Nestin and Pax6 expression in parthenogenetic embryonic stem cells was significantly higher than that in fertilized embryo-derived embryonic stem cells. Thus, our experimental findings indicate that parthenogenetic embryonic stem cells have stronger neuronal differentiation potential than fertilized embryo-derived embryonic stem cells.
