白藜蘆醇作為一種天然的酚化合物,，有預防心血管疾病和抗腫瘤,，以及神經(jīng)保護作用。
胚胎神經(jīng)干細胞存在于中樞神經(jīng)系統(tǒng)中,，可分化為神經(jīng)元和膠質(zhì)細胞,。有研究以胚胎神經(jīng)干細胞移植誘導神經(jīng)退行性疾病動物模型丟失神經(jīng)元再生。
白藜蘆醇是否對胚胎神經(jīng)干細胞產(chǎn)生影響,，從而提高其用于治療神經(jīng)退行性疾病的能力呢,？
發(fā)表于2013年2月第6期《中國神經(jīng)再生研究（英文版）》(Neural Regeneration Research)雜志的一項研究”Effects of resveratrol on hydrogen peroxide-induced oxidative stress in embryonic neural stem cells”表明，白藜蘆醇提高抗氧化酶活性的作用可減輕胚胎神經(jīng)干細胞受到的氧化應激性損害,。
白藜蘆醇拮抗胚胎神經(jīng)干細胞過氧化氫神經(jīng)損害和氧化損傷的實驗流程
實驗旨在觀察白藜蘆醇拮抗過氧化氫神經(jīng)損害和氧化損傷的作用,。過氧化氫可提高胚胎神經(jīng)干細胞過氧化氫酶和谷胱甘肽過氧化物酶、一氧化氮合酶活性,，以及一氧化氮水平,，但對超氧化物歧化酶活性無明顯影響。白藜蘆醇可拮抗過氧化氫誘導的胚胎神經(jīng)干細胞一氧化氮合酶和一氧化氮水平上升,。白藜蘆醇可減輕誘導的胚胎神經(jīng)干細胞核DNA和線粒體DNA損害作用。
研究為白藜蘆醇對抗氧化損傷,，間接提高干細胞移植治療神經(jīng)退行性疾病效果提供了實驗證據(jù),。(生物谷Bioon.com)
DOI: 10.3969/j.issn.1673-5374.2013.06.001
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Effects of resveratrol on hydrogen peroxide-induced oxidative stress in embryonic neural stem cells
Sibel Konyalioglu, Guliz Armagan, Ayfer Yalcin, Cigdem Atalayin, Taner Dagci.
Resveratrol, a natural phenolic compound, has been shown to prevent cardiovascular diseases and cancer and exhibit neuroprotective effects. In this study, we examined the neuroprotective and antioxidant effects of resveratrol against hydrogen peroxide in embryonic neural stem cells. Hydrogen peroxide treatment alone increased catalase and glutathione peroxidase activities but did not change superoxide dismutase levels compared with hydrogen peroxide + resveratrol treatment. Nitric oxide synthase activity and concomitant nitric oxide levels increased in response to hydrogen peroxide treatment. Conversely, resveratrol treatment decreased nitric oxide synthase activity and nitric oxide levels. Resveratrol also attenuated hydrogen peroxide-induced nuclear or mitochondrial DNA damage. We propose that resveratrol may be a promising agent for protecting embryonic neural stem cells because of its potential to decrease oxidative stress by inducing higher activity of antioxidant enzymes, decreasing nitric oxide production and nitric oxide synthase activity, and alleviating both nuclear and mitochondrial DNA damage.
