線粒體功能障礙可能導(dǎo)致與年齡相關(guān)的神經(jīng)退行性疾病，病理狀態(tài)下線粒體的形態(tài)可發(fā)生變化。
一項(xiàng)關(guān)于“Three-dimensional structure of axonal mitochondria reflects the age of drosophila”的研究顯示，用Fiji和Neurolucida軟件可構(gòu)造出神經(jīng)元軸突內(nèi)線粒體的三維圖，直觀的了解到不同年齡階段果蠅軸突內(nèi)線粒體的形態(tài)及分布。通過軸突內(nèi)線粒體形態(tài)、數(shù)目,、大小可推斷線粒體分裂及融合失衡，顯示出軸突線粒體的形態(tài)和數(shù)量隨果蠅日齡的變化而變化的特點(diǎn),。
此研究結(jié)果發(fā)布在《中國神經(jīng)再生研究（英文版）》NEURAL REGENERATION RESEARCH 雜志2013年3月第7期出版的文章上,。（生物谷Bioon.com）
doi:10.3969/j.issn.1673-5374.2013.07.005
PMC:
PMID:
Three-dimensional structure of axonal mitochondria reflects the age of drosophila☆
Honglian Zhu1, 2 , Xiaojiang Sun1
This study aimed to reconstruct a three-dimensional map of axonal mitochondria using Fiji and Neurolucida software, and to observe directly the morphology and distribution of mitochondria in axons of motor neurons in dorsal longitudinal flight muscles of drosophila aged 5 days and 20 days, using electron microscopy. Results indicated that there was no difference in the total area and volume of mitochondria between 5-day-old drosophila and 20-day-old drosophila in all sections, but the ratio of mitochondrial total areas to axon total areas, as well as mitochondrial density of 20-day-old drosophila, was lower than that of 5-day-old drosophila. The number of mitochondria, whose volume was less than 1 000 000 μm3 , and between 1 000 000 μm3 and 10 000 000 μm3 , was higher in 20-day-old drosophila than that in 5-day-old drosophila. The number of mitochondria with a volume between 1 000 000 μm3 and 100 000 000 μm3 was apparently higher than those with a volume less than 1 000 000 μm3 or larger than 100 000 000 μm3 . In addition, the number of mitochondria with a volume more than 100 000 000 μm3 was small; however, the volume was nearly 70% of the total volume in both 5-day-old and 20-day-old drosophila. In contrast, the number of mitochondria with a volume between 1 000 000 μm3 and 10 000 000 μm3 was large, but the volume was less than 30% of the total volume. These experimental findings suggest that changes in mitochondrial morphology and number in motor neurons from the dorsal longitudinal muscle of drosophila are present during different ages.
