最近10年,，對(duì)下丘腦-垂體-性腺軸的深入研究使人們對(duì)此有了進(jìn)一步的認(rèn)識(shí),，發(fā)現(xiàn)了2種新的下丘腦精氨酸-苯丙氨酸酰胺相關(guān)肽kisspeptin和促性腺激素抑制激素，這些物質(zhì)有調(diào)節(jié)生殖軸的作用,。Kisspeptins是下丘腦-垂體-性腺軸最有效的激活劑,，Kisspeptin及其受體GPR54在促性腺激素釋放激素神經(jīng)元中表達(dá)，并調(diào)節(jié)其功能,，可促進(jìn)青春前期促性腺激素釋放激素的釋放和黃體生成素的分泌,。kisspeptin/GPR54系統(tǒng)對(duì)于調(diào)節(jié)性成熟和生殖細(xì)胞的發(fā)育很重要。
土耳其Firat 大學(xué)醫(yī)學(xué)院Haluk Kelestimur教授所在研究團(tuán)隊(duì)以表達(dá)促性腺激素釋放激素的GT1-7細(xì)胞系為模型,，研究發(fā)現(xiàn)精氨酸-苯丙氨酸酰胺相關(guān)肽促性腺激素抑制激素或精氨酸-苯丙氨酸酰胺相關(guān)肽1對(duì)kisspeptins激活的促性腺激素釋放激素神經(jīng)元激活有抑制作用,，這種抑制作用的發(fā)揮不需要降低[Ca2+]i 。說明,，精氨酸-苯丙氨酸酰胺相關(guān)肽3,，1均參與下丘腦-垂體-性腺軸的調(diào)節(jié)作用。相關(guān)文章發(fā)表于2013年6月第18期《中國神經(jīng)再生研究（英文版）》雜志上,。（生物谷 Bioon.com）
生物谷推薦的英文摘要
Neural Regeneration Research DOI: 10.3969/j.issn.1673-5374.2013.18.009
Arg-Phe-amide-related peptides influence gonadotropin-releasing hormone neurons Haluk
Kelestimur, Emine Kacar, Aysegul Uzun, Mete Ozcan, Selim Kutlu
The hypothalamic Arg-Phe-amide-related peptides, gonadotropin-inhibitory hormone and orthologous mammalian peptides of Arg-Phe-amide, may be important regulators of the hypothalamus-pituitary-gonadal reproductive axis. These peptides may modulate the effects of kisspeptins because they are presently recognized as the most potent activators of the hypothalamus-pituitary-gonadal axis. However, their effects on gonadotropin-releasing hormone neurons have not been investigated. In the current study, the GT1–7 cell line-expressing gonadotropin-releasing hormone was used as a model to explore the effects of Arg-Phe- amide-related peptides on kisspeptin activation. Intracellular calcium concentration was quantified using the calcium-sensitive dye, fura-2 acetoxymethyl ester. Gonadotropin-releasing hormone released into the medium was detected via enzyme-linked immunosorbent assay. Results showed that 100 nmol/L kisspeptin-10 significantly increased gonadotropin-releasing hormone levels (at 120 minutes of exposure) and intracellular calcium concentrations. Co-treatment of kisspeptin with 1 μmol/L gonadotropin-inhibitory hormone or 1 μmol/L Arg-Phe-amide-related peptide-1 significantly attenuated levels of kisspeptin-induced gonadotropin-releasing hormone but did not affect kisspeptin-induced elevations of intracellular calcium concentration. Overall, the results suggest that gonadotropin-inhibitory hormone and Arg-Phe-amide-related peptide-1 may have inhibitory effects on kisspeptin-activated gonadotropin-releasing hormone neurons independent of the calcium signaling pathway.
