生物谷報道：1月8日，北京生命科學(xué)研究所周儉民實驗室在《當(dāng)代生物學(xué)》（Current Biology）雜志上發(fā)表題為Pseudomonas syringae Effector AvrPto Blocks Innate Immunity by Targeting Receptor Kinases的文章,。該文報道了丁香假單胞菌（Pseudomonas syringae）效應(yīng)蛋白AvrPto幫助細菌侵染植物的分子機理,。植物依賴包括FLS2和EFR在內(nèi)的受體激酶感應(yīng)細菌的入侵并激活免疫反應(yīng)。該文工作發(fā)現(xiàn),，AvrPto進入植物細胞內(nèi)后直接作用于FLS2和EFR等受體激酶,，阻斷信號傳導(dǎo)，使植物喪失感受細菌的能力,。而在某些抗病番茄中，AvrPto能被胞內(nèi)蛋白激酶Pto識別,，并激活另一條更為強烈的免疫反應(yīng)通路,。該文工作發(fā)現(xiàn)Pto-AvrPto和FLS2-AvrPto作用方式類似,，表明Pto在進化上很可能模擬了受體激酶，作為一個假靶標(biāo)吸引AvrPto,，從而讓植物獲得抗病性,。
向婷婷（博士研究生），宗娜（博士后）,，和鄒燕（北京生命科學(xué)研究所博士畢業(yè)）為本文共同第一作者,。其他參與此項工作的還有：吳勇，張杰（北京生命科學(xué)研究所博士畢業(yè)）,，邢維滿（北京生命科學(xué)研究所博士畢業(yè)）,，李燕，堪撒斯州立大學(xué)的唐曉艷教授,，中科院遺傳發(fā)育所的朱立煌研究員和我所的柴繼杰研究員,。北京生命科學(xué)研究所研究員周儉民博士是本文的通訊作者。
此項研究為科技部863和北京市科委資助課題,，在北京生命科學(xué)研究所完成,。（來源：北京生命科學(xué)研究所）
生物谷推薦原始出處：
Current Biology, Vol 18, 74-80, 08 January 2008
Report
Pseudomonas syringae Effector AvrPto Blocks Innate Immunity by Targeting Receptor Kinases
Tingting Xiang,1,2,5 Na Zong,2,5 Yan Zou,2,3,5 Yong Wu,2 Jie Zhang,2 Weiman Xing,2 Yan Li,2 Xiaoyan Tang,4 Lihuang Zhu,3 Jijie Chai,2 and Jian-Min Zhou2,
1 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
2 National Institute of Biological Sciences, Beijing 102206, China
3 Institute of Genetics, Chinese Academy of Sciences, Beijing 100864, China
4 Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506
Corresponding author
Jian-Min Zhou
[email protected]
Summary
Plants use receptor kinases, such as FLS2 and EFR, to perceive bacterial pathogens and initiate innate immunity. This immunity is often suppressed by bacterial effectors, allowing pathogen propagation. To counteract, plants have evolved disease resistance genes that detect the bacterial effectors and reinstate resistance. The Pseudomonas syringae effector AvrPto promotes infection in susceptible plants but triggers resistance in plants carrying the protein kinase Pto and the associated resistance protein Prf. Here we show that AvrPto binds receptor kinases, including Arabidopsis FLS2 and EFR and tomato LeFLS2, to block plant immune responses in the plant cell. The ability to target receptor kinases is required for the virulence function of AvrPto in plants. The FLS2-AvrPto interaction and Pto-AvrPto interaction appear to share similar sequence requirements, and Pto competes with FLS2 for AvrPto binding. The results suggest that the mechanism by which AvrPto recognizes virulence targets is linked to the evolution of Pto, which, in association with Prf, recognizes the bacterium and triggers strong resistance.
