微生物學(xué)報(bào)Acta Microbiologica Sinica
48(11)：1445~1450; 4 November 2008
洋蔥伯克氏菌基因型的鑒定及其在苜蓿模型上的毒力分析
張立新1, 2,，宋江華3,，謝關(guān)林2 *
（1安徽農(nóng)業(yè)大學(xué)植物保護(hù)學(xué)院，合肥 230036）
（2浙江大學(xué)生物技術(shù)研究所,，杭州 310029）
（3安徽農(nóng)業(yè)大學(xué)園藝學(xué)院,，合肥 230036）
摘要：【目的】證實(shí)來自我國(guó)農(nóng)業(yè)和醫(yī)院環(huán)境中部分洋蔥伯克氏菌的基因型并通過苜蓿植物模型探測(cè)不同基因型對(duì)人體的可能毒力?！痉椒ā坎捎醚笫[伯克氏菌基因型的PCR特異性擴(kuò)增技術(shù)對(duì)來源于根圍,、土壤和醫(yī)院中的57株洋蔥伯克氏菌進(jìn)行了基因型的鑒定，并利用苜蓿植物模型對(duì)這些基因型菌株進(jìn)行了毒力探測(cè),?！窘Y(jié)果】獲得4種不同的基因型，包括基因型Ⅰ,、ⅢA、ⅢB,、Ⅴ和Ⅸ,。來源于醫(yī)院的基因型Ⅰ和ⅢA菌株以及根圍的基因型ⅢB菌株均對(duì)苜蓿幼苗有較強(qiáng)的毒力，其對(duì)苜蓿幼苗的平均發(fā)病率分別達(dá)到69%,、68%和55%,，與農(nóng)田環(huán)境中基因型Ⅴ和Ⅸ對(duì)苜蓿幼苗的發(fā)病率相比,表現(xiàn)出顯著的差異性?！窘Y(jié)論】農(nóng)田環(huán)境中洋蔥伯克菌的基因型在苜蓿模型上的毒力差異大,，根圍的基因型ⅢB菌株對(duì)苜蓿幼苗具有強(qiáng)毒力，其毒力程度接近于醫(yī)院致病基因型ⅢA菌,。
關(guān)鍵詞：洋蔥伯克氏菌,；基因型；苜蓿,；毒力
中圖分類號(hào)：Q933 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：0001-6209 (2008) 11-1445-06
Identification of the Burkholderia cepacia complex genomovars and their virulence in an alfalfa infection model
Lixin Zhang1, 2, Jianghua Song3, Guanlin Xie2*
(1 College of Plant Protection, Anhui Agricultural University, Hefei 230036, China)
(2Insitute of Biotechnology, Zhejiang University, Hangzhou 310029, China)
(3 College of Horticulture, Anhui Agricultural University, Hefei 230036, China)
Abstract: [Objective] To confirm Burkholderia cepacia complex (Bcc) genomovars from agricultural niches and clinical samples, and to evaluate their possible virulence to human body on alfalfa infection model in China. [Methods] A total of 57 Bcc strains were isolated and collected from the rhizosphere, soil and clinical samples in China. The genomovars composition of the Bcc strains was analyzed by species-specific PCR tests, and the virulence of the Bcc strains was tested on alfalfa seedlings. [Results] Four genomovars of the ten genomovars were detected among the Bcc strains, including B. cepacia (genomovarⅠ), B. cenocepacia (genomovarⅢ), B. vietnamiensis (genomovarⅤ) and B. pyrrocinia (genomovar Ⅸ). Bcc genomovarsⅠandⅢA from clinic, and genomovarⅢB from rhizosphere were the most virulent in the alfalfa infection model, and caused symptoms in 69%, 68% and 55% of seedlings, respectively. There were significant variances in the mean percentage of seedlings with symptoms for genomovarsⅠ,ⅢA andⅢB compared to those for genomovarⅤandⅨ. [Conclusion] There was difference in the ability to cause disease in alfalfa for different genomovar strains from agricultural inches. The strains of Bcc genomovarⅢB from rhizosphere were more virulent similar to those of Bcc genomovarⅢA from clinic.
Keywords: Burkholderia cepacia complex; genomovar; alfalfa; virulence
生物谷推薦全文下載：洋蔥伯克氏菌基因型的鑒定及其在苜蓿模型上的毒力分析
更多全文請(qǐng)查看鏈接：http://journals.im.ac.cn
聲明：本文由《微生物學(xué)報(bào)》授權(quán)生物谷 www.bioon.com 網(wǎng)站發(fā)布,，如需轉(zhuǎn)載請(qǐng)直接與中國(guó)科學(xué)院微生物研究所期刊聯(lián)合編輯部聯(lián)系并支付相應(yīng)費(fèi)用，未經(jīng)授權(quán)不得轉(zhuǎn)載,，若轉(zhuǎn)載將付相應(yīng)的法律責(zé)任
