微生物學(xué)報(bào)Acta Microbiologica Sinica
48(11)：1425~1431; 4 November 2008
兩株海洋蛭弧菌的分離及生物學(xué)性質(zhì)
彭宗輝1,，蔡俊鵬1,2,，吳冰3,，袁爾東2*
（1華南理工大學(xué)生物科學(xué)與工程學(xué)院,，廣州 510006）
（華南理工大學(xué),，2輕工與食品學(xué)院,，3分析測(cè)試中心,，廣州 510640）
摘要：【目的】從深圳灣海泥中分離鑒定蛭弧菌,，并對(duì)其生物學(xué)性質(zhì)進(jìn)行初步研究,。【方法】通過(guò)稀釋營(yíng)養(yǎng)肉湯(dilute nutrient broth,，DNB)雙層平板法分離蛭弧菌,，對(duì)所分離的菌株進(jìn)行電鏡形態(tài)觀測(cè)，并進(jìn)行16S rDNA測(cè)序分析,，之后結(jié)合1994年版伯杰氏鑒定細(xì)菌學(xué)手冊(cè)對(duì)菌株進(jìn)行鑒定,，最后通過(guò)生理試驗(yàn)對(duì)其生物學(xué)性質(zhì)進(jìn)行研究?！窘Y(jié)果】從深圳灣海泥中分離出2株蛭弧菌,，分別命名為5#-12和5#-sh06，它們可在20℃~35℃范圍內(nèi)生長(zhǎng),，最適溫度分別是25℃和30℃,；生長(zhǎng)pH范圍6.1~8.6，最適pH均為7.2,；2株蛭弧菌可分別裂解46和48株試驗(yàn)菌,，各占總試驗(yàn)菌株數(shù)（58）的79.3%和82.8%；聯(lián)合2株蛭弧菌,，可裂解56株試驗(yàn)菌,，占總試驗(yàn)菌株數(shù)的96.6%,；同時(shí)，它們一起能將所有試驗(yàn)弧菌裂解,?！窘Y(jié)論】研究結(jié)果揭示了蛭弧菌作為一種生物凈化因子具有極大的潛在應(yīng)用價(jià)值。
關(guān)鍵詞：蛭弧菌,；致病菌,；分離；生物學(xué)性質(zhì)
中圖分類號(hào)：Q939 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：0001-6209 (2008) 11-1425-07
Isolation and characterization of Bdellovibrio strains from the ocean
Zonghui Peng1, Junpeng Cai 1, 2, Bing Wu 3, Erdong Yuan2*
(1College of Bioscience and Engineering, South China University of Technology, Guangzhou 510006, China)
(2College of Light and Food, 3Analytical and Testing Center, South China University of Technology, Guangzhou 510640, China)
Abstract:[ Objective] To isolate and identify the new Bdellovibrio strains from the sea mud of Shenzhen bay and to preliminarily study their biological characteristics. [Methods] We isolated Bdellovibrio strains by DNB(dilute nutrient broth) double-layer plate method. Their 16S rDNAs were sequenced and their morphologies were examined under electron microscope. We identified these strains according to the ninth edition of Bergey's manual of determinative bacteriology. We also studied their biological characteristics through physiological tests. [Results] We isolated 2 strains of Bdellovibrio sp. (5#-12 and 5#-sh06) from sea mud of Shenzhen bay. Both strains grew between 20℃ and 35℃, with 25℃ and 30℃ as optimal temperature for 5#-12 and 5#-sh06, respectively. They grew between pH 6.1 and 8.6, and the opti℃mal pH for both was 7.2. Lysis experiments on 58 strains of pathogens were conducted and the results showed that 5#-12 and 5#-sh06 lysed 46 and 48 strains, corresponding to 79.3% and 82.8% of lysis abilities. Taken both two Bdellovibrio strains together, they lysed 96.6% (56 strains) of tested pathogens and 100% of tested vibrios (39 strains). [Conclusion] The results demonstrated that Bdellovibrio have potential and significant application prospect for elimination of pathogens.
Keywords: Bdellovibrio sp.; pathogens; isolation; biological characteristics
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