Mycosystema
菌 物 學(xué) 報(bào) 15 November 2008, 27(6): 852-862
ISSN1672-6472 CN11-5180Q
2008 Institute of Microbiology, CAS, all rights reserved.
中國禾本科植物內(nèi)生真菌研究4. Epichloë yangzii的人工雜交
亢燕 李偉 紀(jì)燕玲 孫相輝 詹漓暉 于漢壽 王志偉*
南京農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院 南京 210095
摘 要：作者首先研究Epichlo? yangzii在植株上的人工雜交,，明確了供試菌株的交配型,。然后將分離自無子座鵝觀草屬植物的23株"Neotyphodium屬"真菌孢子分別與E. yangzii的子座雜交，其中發(fā)現(xiàn)有21株與E. yangzii（mat-1,，mat-2）雜交不親和,，有2株與E. yangzii（mat-1）雜交親和,。利用tubB基因片段對8株"Neotyphodium屬"真菌菌株進(jìn)行系統(tǒng)發(fā)育分析，結(jié)果表明與E. yangzii雜交親和的2個(gè)菌株和E. yangzii聚為一枝,，而其它6個(gè)菌株形成獨(dú)立的分枝,，進(jìn)一步證實(shí)了這2個(gè)菌株是有時(shí)在宿主植物上不形成子座的E. yangzii。這說明了在宿主植物上的人工雜交是區(qū)別有時(shí)不產(chǎn)子座的E. yangzii和Neotyphodium屬真菌的有效手段,。
關(guān)鍵詞：Epichloë 內(nèi)生真菌,，鵝觀草屬植物，交配型,，子座,，tubB
Grass endophyte researches 4. Mating tests of Epichloë yangzii
KANG Yan LI Wei JI Yan-Ling SUN Xiang-Hui ZHAN Li-Hui YU Han-Shou WANG Zhi-Wei*
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
Abstract: Mating tests between isolates of Epichloë yangzii were conducted and different mating types of isolates were confirmed. Mating tests between stromata of E. yangzii and spermatia obtained from 23 "Neotyphodium" isolates of non-stromata producing Roegneria spp. were also carried on. Two "Neotyphodium" isolates were interfertile with E. yangzii (mat-1), while the remained 21 isolates were not interfertile with E. yangzii (mat-1, mat-2). To further verify the result of mating tests, phylogenetic analysis based on tubB sequences of 8 "Neotyphodium" isolates, including 2 cross-fertile isolates, were conducted. The 2 cross-fertile isolates clustered with E. yangzii were significantly separated from the clade of the remained six isolates, indicating they should be E. yangzii. These evidences support that the mating test is an effective way to distinguish non-stromata producing E. yangzii from Neotyphodium sp.
Key words: epichlo? endophyte, Roegneria spp., mating type, stromata, tubB
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