菌物學(xué)報 15 July 2009, 28(4):496～503
隴南地區(qū)不同海拔區(qū)域內(nèi)小麥條銹菌群體遺傳多樣性的分析
陸寧海1,2 王建鋒1,2 詹剛明1 黃麗麗1 康振生1,2*
1西北農(nóng)林科技大學(xué)植物保護(hù)學(xué)院 楊凌 712100
2西北農(nóng)林科技大學(xué) 陜西省農(nóng)業(yè)分子生物學(xué)重點實驗室 楊凌 712100
摘 要：小麥條銹病是世界范圍內(nèi)小麥上最重要的流行性病害之一,，可造成嚴(yán)重的產(chǎn)量損失,。隴南地區(qū)是我國小麥條銹菌主要越夏易變區(qū)和新小種發(fā)源地,，了解該地區(qū)不同海拔高度區(qū)域內(nèi)條銹菌遺傳多樣性有重要意義,。本研究采用TP-M13-SSR 熒光標(biāo)記技術(shù)對11個種群330個小麥條銹菌分離株基因組DNA進(jìn)行了SSR標(biāo)記分析。不同海拔區(qū)域的條銹菌遺傳多樣性有明顯的差異,，高山區(qū)的遺傳多樣性比較豐富,，半山區(qū)次之，川道區(qū)相對比較低,。不同生態(tài)區(qū)域內(nèi),，小麥條銹菌群體遺傳分化程度不同，高山區(qū)和半山區(qū)遺傳分化程度大,，基因流小,，川道區(qū)群體遺傳分化程度比較小，基因流大,。來自不同海拔區(qū)域的菌系具有相同的基因型,， 這一結(jié)果從分子水平證明了在隴南地區(qū)小麥條銹菌在山區(qū)與川地之間存在廣泛的菌源交流, 可就地完成周年循環(huán)。
關(guān)鍵詞：小麥條銹菌,，遺傳分化,，海拔，SSR標(biāo)記
SSR analysis of population genetic diversity of Puccinia striifornis f. sp. tritici in different altitude regions of Longnan, Gansu
LU Ning-Hai1, 2 WANG Jian-Feng1, 2 ZHAN Gang-Ming1 HUANG Li-Li1 KANG Zhen-Sheng1, 2*
1College of Plant Protection, Northwest Agriculture & Forestry University, Yangling 712100, China
2Shaanxi Key Laboratory of Molecular Biology, Northwest Agriculture & Forestry University, Yangling 712100, China
Abstract: Stripe rust (or yellow rust) of wheat (Triticum aestivum), caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. It is very important to research the population genetic diversity of P. striifornis f. sp. tritici in different altitude regions of Longnan, one of the largest and the most important over-summering areas in China. Using the TP-M13-SSR, population genetic diversity and variation of P. striiformis f. sp. tritici, containing 330 isolates 11 collections from different altitude area of Longnan, were investigated. The important conclusion is that P. striifornis f. sp. tritici populations from different altitude areas were genetically different. Genetic diversity for the pathogen population from high mountainous area is much higher than that from mountainside area or lowlands. The genetic differentiation for the populations obtained from high mountain and mountainside areas was higher than that of the population from the lowlands, suggesting the limited gene flow in mountain area, and extensive gene exchange in lowlands. Our molecular data confirm that the stripe rust pathogen is able to exchange between lowland areas and high mountain areas in the region.
Key words: stripe rust of wheat, genetic differentiation, SSR marker, altitude
生物谷推薦全文下載：隴南地區(qū)不同海拔區(qū)域內(nèi)小麥條銹菌群體遺傳多樣性的分析
更多全文請查看鏈接：http://journals.im.ac.cn
聲明：本文由《微生物學(xué)報》授權(quán)生物谷 www.bioon.com 網(wǎng)站發(fā)布,，如需轉(zhuǎn)載請直接與中國科學(xué)院微生物研究所期刊聯(lián)合編輯部聯(lián)系并支付相應(yīng)費用,，未經(jīng)授權(quán)不得轉(zhuǎn)載，若轉(zhuǎn)載將付相應(yīng)的法律責(zé)任,。
