菌物學(xué)報 15 July 2009, 28(4):504～511
銀杏葉部內(nèi)生真菌多樣性的研究
杜少康 陳雙林* 林岱 吳鳴謙 王梅霞
南京師范大學(xué)生命科學(xué)學(xué)院 南京 210046
摘 要：為了解我國特有植物銀杏的葉部組織中內(nèi)生真菌菌群的多樣性和演替變化,，分別于春、夏,、秋季從江蘇和山東兩省的五個主要銀杏栽培地采集健康葉片進(jìn)行了內(nèi)生真菌的研究,。結(jié)果表明：所分離出的1971株內(nèi)生真菌可歸于32屬，以交鏈孢屬Alternaria（18.01%）,、葉點霉屬Phyllosticta（14.66%）和絲核菌屬Rhizoctonia（14.56%）為優(yōu)勢菌群,。在內(nèi)生真菌的類群及組成上存在著部位、地區(qū)以及季節(jié)的差異性,，也呈現(xiàn)一定的規(guī)律性,。多樣性指數(shù)的計算反映出所獲得的銀杏葉部內(nèi)生真菌菌群具有不同的豐富度及均勻度，同時有一定的相似性,。
關(guān)鍵詞：分離率,，分離頻率,，優(yōu)勢菌群，多樣性指數(shù),，相似性系數(shù)
Diversity of endophytic fungi in leaves of Ginkgo biloba
DU Shao-Kang CHEN Shuang-Lin* LIN Dai WU Ming-Qian WANG Mei-Xia
College of Life Science, Nanjing Normal University, Nanjing 210046, China
Abstract: Diversity of endophytic fungi in leaves of Ginkgo biloba was studied in order to understand diversity state and succession change of endophytic fungal communities in this endemic plant. Healthy leaf samples were collected from five main planting locations of G. biloba in Jiangsu and Shandong provinces separately in spring, summer and autumn. A total of 1971 strains of endophytic fungi was isolated. As a result of morphological identification 32 genera were obtained, of which Alternaria (amounting to 18.01% of the total), Phyllosticta (14.66%) and Rhizoctonia (14.56%) were dominant. The distribution and composition of endophytic fungi varied with plant organs colonized, localities and seasons, but they assumed regularity to a certain extent. Populations of endophytic fungi in different parts of the leaves indicated different abundance and uniformity but assumed similarity to a certain extent.
Key words: isolation rate, isolation frequency, dominant population, diversity index, similarity coefficient
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