微生物學(xué)通報(bào) JAN 20,2010,37(1):30～36

一株沙門(mén)氏菌拮抗菌的篩選及其在污染土壤原位修復(fù)中的應(yīng)用

宋德顯1,2,3  尹睿1,2*  林先貴1,2  王一明1,2  張華勇1,2  王紀(jì)杰1,2

(1. 中國(guó)科學(xué)院南京土壤研究所土壤與農(nóng)業(yè)可持續(xù)發(fā)展國(guó)家重點(diǎn)實(shí)驗(yàn)室  江蘇 南京  210008)

(2. 中國(guó)科學(xué)院南京土壤研究所-香港浸會(huì)大學(xué)土壤與環(huán)境聯(lián)合開(kāi)放實(shí)驗(yàn)室  江蘇 南京  210008)

(3. 中國(guó)科學(xué)院研究生院  北京  100049)

摘  要: 利用無(wú)菌濾紙片平板法從沙門(mén)氏菌(Salmonella)污染土壤中篩選到一株有效拮抗沙門(mén)氏菌的細(xì)菌A45, 通過(guò)形態(tài)學(xué),、革蘭氏染色和16S rDNA序列同源性分析鑒定為產(chǎn)堿桿菌(Alcaligenes sp.)。溫室土培試驗(yàn)和田間原位試驗(yàn)結(jié)果都發(fā)現(xiàn), 利用該菌株制備的沙門(mén)氏菌拮抗菌劑能顯著降低土壤中沙門(mén)氏菌數(shù)量(P < 0.05), 與對(duì)照相比土壤中沙門(mén)氏菌數(shù)量下降2 3個(gè)數(shù)量級(jí), 表明該拮抗細(xì)菌可應(yīng)用于沙門(mén)氏菌污染土壤的修復(fù),。

關(guān)鍵詞: 沙門(mén)氏菌, 拮抗菌, 土壤污染, 微生物修復(fù), 產(chǎn)堿菌屬

Screening of an Antagonistic Bacterial Strain Against Salmonella and Its Application in In-site

Bioremediation of Salmonella-contaminated Soils

SONG De-Xian1,2,3  YIN Rui1,2*  LIN Xian-Gui1,2  WANG Yi-Ming1,2

ZHANG Hua-Yong1,2  WANG Ji-Jie1,2

(1. State Key Laboratory of Soil and Sustain able Agriculture, Institute of Soil Science, Chinese Academy of

Sciences, Nanjing, Jiangsu 210008, China)

(2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science and Hongkong

Baptist University, Nanjing, Jiangsu 210008, China)

(3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China)

Abstract: A bacterial strain, A45, with antagonistic activity against Salmonella was isolated from Salmo-nella-contaminated soils, which was identified as Alcaligenes sp. by checking the individual morphology, colony characteristics and 16S rDNA sequencing. The inhibiting effects of A45 to Salmonella in soils were investigated by field and greenhouse pot experiments respectively. The amounts of Salmonella in soils de-creased up to three orders of magnitude after spraying A45 into soils both under greenhouse and field condi-tions. It suggested that it might be an effective, economical and environmentally friendly approach to apply A45 for in-site remediation of pathogens-contaminated soils.

Keywords: Salmonella, Antagonistic bacterium, Soil pollution, Bioremediation, Alcaligenes sp.

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