微生物學通報 JAN 20,2010,37(1):30～36
一株沙門氏菌拮抗菌的篩選及其在污染土壤原位修復中的應用
宋德顯1,2,3 尹睿1,2* 林先貴1,2 王一明1,2 張華勇1,2 王紀杰1,2
(1. 中國科學院南京土壤研究所土壤與農業(yè)可持續(xù)發(fā)展國家重點實驗室 江蘇 南京 210008)
(2. 中國科學院南京土壤研究所-香港浸會大學土壤與環(huán)境聯(lián)合開放實驗室 江蘇 南京 210008)
(3. 中國科學院研究生院 北京 100049)
摘 要: 利用無菌濾紙片平板法從沙門氏菌(Salmonella)污染土壤中篩選到一株有效拮抗沙門氏菌的細菌A45, 通過形態(tài)學、革蘭氏染色和16S rDNA序列同源性分析鑒定為產堿桿菌(Alcaligenes sp.),。溫室土培試驗和田間原位試驗結果都發(fā)現(xiàn), 利用該菌株制備的沙門氏菌拮抗菌劑能顯著降低土壤中沙門氏菌數(shù)量(P < 0.05), 與對照相比土壤中沙門氏菌數(shù)量下降2 3個數(shù)量級, 表明該拮抗細菌可應用于沙門氏菌污染土壤的修復,。
關鍵詞: 沙門氏菌, 拮抗菌, 土壤污染, 微生物修復, 產堿菌屬
Screening of an Antagonistic Bacterial Strain Against Salmonella and Its Application in In-site
Bioremediation of Salmonella-contaminated Soils
SONG De-Xian1,2,3 YIN Rui1,2* LIN Xian-Gui1,2 WANG Yi-Ming1,2
ZHANG Hua-Yong1,2 WANG Ji-Jie1,2
(1. State Key Laboratory of Soil and Sustain able Agriculture, Institute of Soil Science, Chinese Academy of
Sciences, Nanjing, Jiangsu 210008, China)
(2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science and Hongkong
Baptist University, Nanjing, Jiangsu 210008, China)
(3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
Abstract: A bacterial strain, A45, with antagonistic activity against Salmonella was isolated from Salmo-nella-contaminated soils, which was identified as Alcaligenes sp. by checking the individual morphology, colony characteristics and 16S rDNA sequencing. The inhibiting effects of A45 to Salmonella in soils were investigated by field and greenhouse pot experiments respectively. The amounts of Salmonella in soils de-creased up to three orders of magnitude after spraying A45 into soils both under greenhouse and field condi-tions. It suggested that it might be an effective, economical and environmentally friendly approach to apply A45 for in-site remediation of pathogens-contaminated soils.
Keywords: Salmonella, Antagonistic bacterium, Soil pollution, Bioremediation, Alcaligenes sp.
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