微生物學(xué)通報(bào) JAN 20,2010,37(1):85～90
一株中度嗜鹽細(xì)菌whb45的鑒定及其抗菌與抗腫瘤活性篩選
陳雷1,2,3 王光玉2 卜同1,3 張?jiān)时? 劉明1 張軍4 林秀坤1*
(1. 中國科學(xué)院海洋研究所實(shí)驗(yàn)海洋生物學(xué)重點(diǎn)實(shí)驗(yàn)室 山東 青島 266071)
(2. 哈爾濱工業(yè)大學(xué)(威海)海洋學(xué)院 山東 威海 264209)
(3. 中國科學(xué)院研究生院 北京 100039)
(4. 山東省醫(yī)藥工業(yè)設(shè)計(jì)院 山東 濟(jì)南 250013)
摘 要: 從鹽場中分離鑒定中度嗜鹽細(xì)菌并對其潛在的抗菌和抗腫瘤活性進(jìn)行評價(jià),。從山東威海的鹿道口鹽場分離嗜鹽細(xì)菌, 對菌株whb45進(jìn)行形態(tài)學(xué)和生理生化特性研究, 測定其16S rRNA序列并通過同源性比對進(jìn)行系統(tǒng)發(fā)育分析, 采用抗菌和細(xì)胞毒模型進(jìn)行活性篩選,。試驗(yàn)結(jié)果表明, 菌株whb45為中度嗜鹽細(xì)菌, whb45與Halobacillus trueperi在形態(tài)和生理生化特征方面最接近, 16S rRNA序列相似性為99%,。whb45的粗提物對多種細(xì)菌,、真菌和腫瘤細(xì)胞的生長都具有較強(qiáng)的抑制作用, 可以作為發(fā)現(xiàn)生物活性物質(zhì)的潛在的新來源,。
關(guān)鍵詞: 中度嗜鹽細(xì)菌, 鑒定, 喜鹽芽孢桿菌屬, 抗菌活性, 抗腫瘤活性
Identification of a Moderately Halophilic Bacterium whb45 and Screening of Its Antimicrobial and Antitumor Activity
CHEN Lei1,2,3 WANG Guang-Yu2 BU Tong1,3 ZHANG Yun-Bin2 LIU Ming1
ZHANG Jun4 LIN Xiu-Kun1*
(1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China)
(2. College of Marine Science, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China)
(3. Graduate School, Chinese Academy of Sciences, Beijing 100039, China)
(4. Shandong Province Design Institute of Pharmaceutical Industry, Jinan, Shandong 250013, China)
Abstract: The aim of this study was to isolate and identify moderately halophilic bacteria from solar saltern and to investigate their potential antimicrobial and antitumor activities. Halophilic bacteria were isolated from Ludaokou Solar Saltern in Weihai of Shandong Province. The identification of the strain whb45 was determined by their morphological characteristics, physiological and biochemical tests and phylogenetic analysis based on 16S rRNA sequence comparison. The antimicrobial activity and cytotoxic activity were screened. Our results showed that the strain whb45 was belonged to moderately halophilic bacterium. Phylogenetic analysis showed the strain whb45 had a 99% homology with Halobacillus trueperi. The crude ex-tract from whb45 showed stronger antimicrobial activities which could inhibit the growth of several bacteria and fungi. MTT analysis revealed that the strain possessed potent anticancer activity with the stronger in-hibitory effect on several human cancer cells. Our results suggested that the moderately halophilic bacterium whb45 may be developed as a potential new source for the discovery of novel bioactive substances.
Keywords: Moderately halophilic bacteria, Identification, Halobacillus, Antimicrobial activity, Antitumor activity
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