微生物學通報 MAR 20,2010,37(3):375～380
一株新的α-糖苷酶抑制劑生產(chǎn)菌株PW0852的初步研究
白芳1 張奇1 彭臻臻2 齊西珍1 白鋼1,2*
(1. 南開大學藥學院 天津 300071)
(2. 南開大學生命科學學院 天津 300071)
摘 要: 從土壤中分離并篩選得到了一株α-糖苷酶抑制劑生產(chǎn)菌PW0852,。根據(jù)形態(tài)特征、培養(yǎng)特征,、生理生化特征及16S rRNA序列相似性分析等多相分類方法, 初步判定菌株PW0852為淡紫灰鏈霉菌(Streptomyces lavendulae),。該菌經(jīng)10 L發(fā)酵罐水平發(fā)酵, 發(fā)酵液中可積累一定量的α-糖苷酶抑制劑。采用膜過濾技術,、離子交換樹脂吸附及冷凍干燥等方法, 從PW0852發(fā)酵液中分離獲得混合型α-糖苷酶抑制劑GIB852,。GIB852對人類胰腺α-淀粉酶和α-麥芽糖酶都有明顯的抑制作用, 其對麥芽糖酶的抑制性比同劑量的市售雙糖酶抑制劑米格列醇強28.7倍, 而對α-淀粉酶的抑制性較弱。通過小鼠糖耐量實驗, 發(fā)現(xiàn)α-糖苷酶抑制劑GIB852對哺乳動物餐后高血糖的形成有明顯改善作用, 具有開發(fā)為糖尿病藥物的潛力,。
關鍵詞: 淡紫灰鏈霉菌, 多相分類, 發(fā)酵, α-糖苷酶抑制劑
Study of a Novel Strain PW0852 Producing α-Glucosidase Inhibitor
BAI Fang1 ZHANG Qi1 PENG Zhen-Zhen2 QI Xi-Zhen1 BAI Gang1,2*
(1. College of Pharmacy, Nankai University, Tianjin 300071, China)
(2. College of Life Sciences, Nankai University, Tianjin 300071, China)
Abstract: Actinomycete strain PW0852 with high production of α-glucosidase inhibitor was isolated from soil samples. Based on the results of morphological, physiological, chemotaxonomic characteristics and 16S rRNA analysis, strain PW0852 was placed within the genus Streptomyces and identified as the species Streptomyces lavendulae. After fermentation in a 10 L fermentor, α-glucosidase inhibitors were accumulated in the harvested broth of strain PW0852. The mixed-type α-glucosidase inhibitor GIB852 we obtained was isolated and purified after ultra-filtration, nano-filtration, resin-adsorption and desiccation. GIB852 could ef-ficaciously inhibit human pancreatic α-amylase and α-maltase glucoamylase. The inhibition of maltase is stronger than the same dose of the commercial α-glucosidase inhibitor miglitol 28.7 times, while the inhibi-tion of α-amylase is weak. GIB852 could significantly depress blood glucose levels in mammalian systems and be developed towards a possible therapeutic agent for diabetes.
Keywords: Streptomyces lavendulae, Polyphasic taxonomy, Fermentation, α-glucosidase inhibitor
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