2010年10月, 北京生命科學(xué)研究所周儉民實(shí)驗(yàn)室在Molecular Plant-Microbe Interactions雜志發(fā)表題為“FLS2, but not BAK1, is a target of the Pseudomonas syringae effector AvrPto.”的文章。
在抵御外界微生物侵染的過程中,，植物細(xì)胞表面的受體激酶通過激活寄主的先天免疫來發(fā)揮重要的防御功能,。而丁香假單胞菌分泌的三型效應(yīng)蛋白AvrPto卻能夠抑制這種先天免疫,，從而幫助細(xì)菌致病。周儉民實(shí)驗(yàn)室在2008年發(fā)表的工作表明AvrPto是通過直接攻擊植物細(xì)胞表面受體激酶FLS2及EFR來實(shí)現(xiàn)毒性功能的,。之后一篇文章報(bào)道AvrPto更有可能是攻擊了FLS2的共同受體BAK1,，而非FLS2。AvrPto的真實(shí)靶點(diǎn)因此而存在爭(zhēng)議,。本論文通過大量嚴(yán)格的生化試驗(yàn)以及遺傳證據(jù),，進(jìn)一步證明AvrPto在植物細(xì)胞中作用于受體激酶FLS2，而不是BAK1,，澄清了爭(zhēng)議。
北京生命科學(xué)研究所博士生向婷婷為本文的第一作者,，論文的其他作者還有NIBS的張杰和宗娜博士,，中科院遺傳與發(fā)育所陳金鋒博士生和陳明生博士等。周儉民博士為本文的通訊作者,。此項(xiàng)研究由科技部863和北京市科委資助,，在北京生命科學(xué)研究所完成。（生物谷Bioon.com）
生物谷推薦英文摘要：
Mol Plant Microbe Interact. PMID:20923364
FLS2, but not BAK1, is a target of the Pseudomonas syringae effector AvrPto.
Xiang T, Zong N, Zhang J, Chen J, Chen M, Zhou J.
Plant cell surface-localized receptor kinases such as FLS2, EFR, and CERK1 play a crucial role in detecting invading pathogenic bacteria. Upon stimulation by bacterium-derived ligands, FLS2 and EFR interact with BAK1, a receptor-like kinase, to activate immune responses. A number of Pseudomonas syringae effector proteins are known to block immune responses mediated by these receptors. Previous reports suggested that the P. syringae effector AvrPto could target both FLS2 and BAK1 to inhibit plant defenses. Here we provide new evidence further supporting that FLS2, but not BAK1, is targeted by AvrPto in plants. The AvrPto-FLS2 interaction prevented the phosphorylation of BIK1, a down-stream component of the FLS2 pathway.
