微生物學通報 OCT 20,2010,37(10):1475～1480
生物氧化產堿去除半纖維素水解物中的有機酸
劉勝男1,2 覃香香1 張厚瑞1* 蔡愛華1 林蘭英2
(1. 中國科學院廣西植物研究所 廣西 桂林 541006)
(2. 廣西師范大學生命科學學院 廣西 桂林 541004)
摘 要: 盔形畢赤酵母Pichia galeiforms B-10對半纖維素水解物中的有機酸具有良好的降解活性, 影響它脫酸活性的最主要因素是水解物初始pH,。將半纖維素水解物初始pH值調節(jié)至5.0以上而無需其他處理, Pichia galeiforms B-10便可發(fā)揮良好的脫酸發(fā)酵性能,。Pichia galeiforms B-10代謝有機酸鹽可產生堿性物質, 使水解液pH升高。在 pH值＞5.0的條件下, 只要調節(jié)補酸(補加低pH值水解物)的速率與代謝耗酸速率相平衡, 發(fā)酵體系即可始終處于有利于酵母快速代謝有機酸的高pH環(huán)境。這種生物氧化產堿連續(xù)脫酸發(fā)酵方式, 可有效降低中和半纖維素水解物的外加用堿量, 具有降低成本, 減少新污染物的優(yōu)勢,。
關鍵詞: 半纖維素水解物, 生物氧化產堿, 有機酸
Removal of Organic Acid from Hemicellulose Hydrolysate by Bio-oxidation to Produce Alkali
LIU Sheng-Nan1,2 QIN Xiang-Xiang1 ZHANG Hou-Rui1* CAI Ai-Hua1
LIN Lan-Ying2
(1. Guangxi Institute of Botany, Chinese Academy of Science, Guilin, Guangxi 541006, China)
(2. Life Science College, Guangxi Normal University, Guilin, Guangxi 541004, China)
Abstract: Pichia galeiforms B-10 has a good ability to degrade organic acid in hemicellulose hydrolysate, and this depickling activity of the strain is mainly affected by the initial pH value of hydrolysate. If the initial pH value of hemicellulose hydrolysate was adjusted above 5.0, Pichia galeiforms B-10 would ex-hibit an excellent capability of depickling fermentation without any other treatment. During the metabo-lism of organic acid salt by Pichia galeiforms B-10, alkali products were generated and this caused the raising of pH value. Therefore, under the condition of pH > 5.0, if only the acid supplying speed (by add-ing hydrolysate with low pH value) was adjusted to be in balance with the acid metabolizing speed, the fermentation system would always be kept in a high pH circumstance which was advantageous to the me-tabolism of organic acid by yeast. This mode, by using bio?oxidation to produce alkali for the continuous depickling fermentation, effectively reduced the addition of alkali during the neutralization of hemicellu-lose hydrolysate, and finally lowered the processing cost and generated less pollutant.
Keywords: Hemicellulose hydrolysate, Bio-oxidation to produce alkali, Organic acid
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