培養(yǎng)法和免培養(yǎng)法聯(lián)合檢測(cè)油藏環(huán)境烴降解菌和產(chǎn)甲烷菌群多樣性
李輝1 林匡飛1 牟伯中2* 張衛(wèi)1 顧繼光3 李洋洋4
(1. 華東理工大學(xué)資源與環(huán)境工程學(xué)院 國(guó)家環(huán)境保護(hù)化工過(guò)程環(huán)境風(fēng)險(xiǎn)評(píng)價(jià)與控制重點(diǎn)實(shí)驗(yàn)室 上海 200237)
(2. 華東理工大學(xué)應(yīng)用化學(xué)研究所 生物反應(yīng)器國(guó)家重點(diǎn)實(shí)驗(yàn)室 上海 200237)
(3. 暨南大學(xué)生命科學(xué)技術(shù)學(xué)院 廣東 廣州 510632)
(4. 上?；ぱ芯吭?nbsp; 上海 200062)
摘 要: 烴降解菌和產(chǎn)甲烷菌是油藏環(huán)境微生物生態(tài)系統(tǒng)中重要的功能菌群, 采用DGGE和FISH方法分析了不同油藏樣品中兩類菌群的多樣性和產(chǎn)甲烷活性。DGGE結(jié)果表明, 不同水樣的alkB基因多樣性相差較大, 而且注水井條帶明顯多于采油井,。FISH結(jié)果表明, 油藏水樣中產(chǎn)甲烷菌含量明顯高于烴降解菌, 且兩者空間分布的位置較近; 說(shuō)明油藏環(huán)境中烴降解菌和產(chǎn)甲烷菌結(jié)成一定的相互關(guān)系,。富集培養(yǎng)表明, 勝利油田產(chǎn)出液接種物培養(yǎng)130 d后, 石油烴降解率達(dá)到50%以上, 產(chǎn)甲烷的最大速率達(dá)到1.57 10?2 mmol/(L·d)。利用分子生物學(xué)方法分析油藏環(huán)境功能菌群的多樣性, 可以為開(kāi)展微生物采油技術(shù)的應(yīng)用提供有用信息,。
關(guān)鍵詞: 油藏, 分子生態(tài), 微生物多樣性, DGGE指紋圖譜, FISH雜交
英文摘要：
微生物學(xué)通報(bào) 2011,，38（1）：21-28
Culture-dependent and culture-independent characterization of alkane-degrading bacteria and methanogens consortium diversity associated with petroleum reservoir
LI Hui1 LIN Kuang-Fei1 MU Bo-Zhong2* ZHANG Wei1 GU Ji-Guang3 LI Yang-Yang4
(1. State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China)
(2. State Key Laboratory of Bioreactor Engineering, Institute of Applied Chemistry, East China University of Science and
Technology, Shanghai 200237, China)
(3. College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China)
(4. Shanghai Research Institute of Chemical Industry, Shanghai 200062, China)
Abstract: The alkane-degrading bacterium and methanogenic were the important functional popula-tions in oil reservoirs and their diversities were investigated by FISH and DGGE, respectively. The DGGE profiles showed great difference between the alkB genes in samples. And the major bands de-tected in injection wells were obviously more than them in production wells. The FISH results indicated that the quantity of archaea was much more than bacteria in the samples and they had close spatial dis-tribution in situ. The results indicated that alkane-degrading bacterium and methanogenic populations were diverse in oil reservoirs and formed compact aggregates in situ. The incubation of Shengli oilfield production water degraded more than 50% residual oil during 130 d culture, with the production rate of methane reaching up to 1.57 10?2 mmol/(L·d). Molecular analysis of the functional populations of mi-crobial community in oilfield will provide the information for better application of microbial enhanced oil recovery.
Keywords: Petroleum reservoir, Molecular ecology, Microbial diversity, DGGE, FISH
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