洋產(chǎn)電菌Shewanella marisflavi EP1的脫色特性
鄒聰慧1  徐方成2*  陳新華3
(1. 廈門大學海洋與環(huán)境學院  福建 廈門  361005)
(2. 廈門大學化學化工學院  福建 廈門  361005)
(3. 國家海洋局第三海洋研究所 海洋生物遺傳資源重點實驗室  福建 廈門  361005)

摘  要: 以一株新篩選得到的海洋產(chǎn)電菌Shewanella marisflavi EP1作為實驗材料, 研究了該菌株關(guān)于偶氮,、蒽醌,、三苯基甲烷等染料的脫色能力及脫色機制,。結(jié)果表明, 該菌株對這些染料均具有較好的脫色能力, 最高脫色容量達到925 mg染料/(g細胞干重·d),。EP1能利用葡萄糖,、蔗糖,、木糖,、乳酸,、甲酸,、檸檬酸等多種碳源將單偶氮染料麗春紅2R脫色,。脫色的pH、溫度和NaCl濃度范圍分別是: pH 6?10,、15 °C?40 °C,、0?8%。最優(yōu)脫色條件: 乳酸, pH 8,、35 °C,、1%?2% NaCl, 10 h內(nèi)脫色率高達99.95%。分光光譜結(jié)果表明, 在0-8% NaCl濃度范圍內(nèi)EP1脫色機制為降解脫色,。

關(guān)鍵詞: 產(chǎn)電菌, Shewanella marisflavi EP1, 偶氮染料, 生物脫色

英文摘要：

Decolorization of dyes by a current-producing bacterium Shewanella marisflavi EP1 isolated from sea sediments
ZOU Cong-Hui1  XU Fang-Cheng2*  CHEN Xin-Hua3
(1. College of Oceanography and Environmental Science, Xiamen University, Xiamen, Fujian 361005, China)
(2. College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, China)
(3. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian 361005, China)

Abstract: In this study, we investigated a new current-producing strain, Shewanella marisflavi EP1 isolated from costal sediments of Xiamen, its decolorization capability and mechanism of several types of dyes such as azo, anthraquinone and triphenyl methane. The results indicated that these dyes could be decolorized efficiently and the maximum capacity was 925 mg/(g cell dry weight·d) of Malachite Green. Strain EP1 could utilize various kinds of carbon sources, such as glucose, sucrose, xylose, lac-tate, formate and citrate for the decolorization of Xylidine Ponceau 2R. EP1 was active for decoloriza-tion in wide ranges of pH (6?10), temperture (15 °C?40 °C), and NaCl concentations (0?8%). The op-timal conditions were pH 8.0, 30 °C, 1%?2% NaCl and lactate as carbon source, with a highest decol-orization rate of 99.95% within 10 h. UV-vis absorption spectra demonstrated that the mechanism of the azo dye was degradation in the range of 0?8% NaCl.

Keywords: Current-producing bacteria, Shewanella marisflavi EP1, Azo dye, Biodecolorization

生 物 谷推薦全文下載