鹽堿土壤PAHs降解菌的篩選鑒定及其降解特性
宋立超1,2 李培軍2 劉宛2 肖亦農(nóng)1 張玉龍1*
(1. 沈陽農(nóng)業(yè)大學(xué)土地與環(huán)境學(xué)院 遼寧 沈陽 110866)
(2. 中國科學(xué)院沈陽應(yīng)用生態(tài)研究所 遼寧 沈陽 110016)
摘 要: 采用富集培養(yǎng)的方法, 從天津大港油田PAHs污染鹽堿化土壤中分離出一株能以菲,、芘為唯一碳源和能源的優(yōu)勢菌TJB5,。經(jīng)形態(tài)觀察和16S rDNA序列分析結(jié)果表明, 該菌株為成團泛菌(Pantoea agglomerans)。采用液體培養(yǎng)的方法, 研究了pH,、鹽度,、菲芘的初始濃度對TJB5菌株降解菲芘效果的影響, 確定了最佳降解條件,。結(jié)果表明, 該菌對菲、芘的降解具有較廣泛的pH,、鹽度范圍和良好的降解效果,。在菲、芘濃度分別為50 mg/L,、pH 6.8-9.5,、鹽度2%-3%、溫度30 °C條件下, 接種15 d后菲降解率在93.3%以上, 芘降解率在20%以上,。
關(guān)鍵詞: 鹽堿化, 多環(huán)芳烴降解菌, 16S rDNA, 降解特性
生物谷推薦英文摘要：
Isolation, identification and degradation characteristics of a PAHs-degrading bacteria from salt-alkaline soil
SONG Li-Chao1,2 LI Pei-Jun2 LIU Wan2 XIAO Yi-Nong1 ZHANG Yu-Long1*
(1. College of Land and Environment, Shenyang Agricultural University, Shenyang, Liaoning 110866, China)
(2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China)
Abstract: A predominant PAHs-degrading strain TJB5 was isolated from PAHs-contaminated salt-alkaline soil in Dagang Oilfield of Tianjin, which can use phenanthrene and pyrene as sole carbon source for growth in the selective culture medium. Strain TJB5 was identified as Pantoea agglomerans according to the results of morphology and the phyogenetical analyses of 16S rDNA sequence. The ef-fect of initial concentration of phenanthrene and pyrene, inoculating pH and salinity on degradation ef-ficiencies was investigated. The optimal degradations were determined. The results indicated that the degrading characteristics of the strain were fine. More than 93.3% of phenanthrene and 20% of pyrene in the medium were degraded in the liquid medium with 50 mg/L phenanthrene and pyrene, pH 6.8?9.5 and salinity ranging from 2% to 3% after 15 days at 30 °C.
Keywords: Salinization, PAHs-degrading strain, 16S rDNA, Biodegradation characteristics
