一株抗香蕉枯萎病內(nèi)生細(xì)菌的分離鑒定及其抗病促生作用
陳博1,2Δ 朱軍1Δ 孫前光1 鄭業(yè)輝3 黃惠琴1 鮑時(shí)翔1*
(1. 中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶生物技術(shù)研究所 海南 海口 571101)
(2. 海南大學(xué) 海南 ?？?nbsp; 570228)
(3. 海南正維爾生物科技有限公司 海南 ?？?nbsp; 571101)
摘 要: 從巴西蕉中分離到一株能夠明顯抑制尖孢鐮刀菌古巴專化型4號(hào)生理小種(FOC4)的內(nèi)生細(xì)菌DB09208, 通過生理生化,、形態(tài)特征及16S rDNA序列同源性比較, 初步認(rèn)為它和類芽孢桿菌(Paenibacillus)比較接近,。該菌株能夠顯著抑制FOC4菌絲生長(zhǎng), 抑菌率為74.09%。盆栽試驗(yàn)表明, 該菌株能夠抑制香蕉枯萎病的發(fā)生, 防治效果達(dá)為65.2%, 該菌同時(shí)能顯著促進(jìn)香蕉苗生根, 促生效果與植物生長(zhǎng)素IAA相似, 顯示出良好的開發(fā)前景,。
關(guān)鍵詞: 香蕉枯萎病, 內(nèi)生細(xì)菌, 16S rDNA, 分離鑒定
生物谷推薦英文摘要：
A bacterial endophyte from banana: its isolation,identification, activity to Fusarium Wilt and PGPR effect to banana seedlings
CHEN Bo1,2Δ ZHU Jun1Δ SUN Qian-Guang1 ZHENG Ye-Hui3 HUANG Hui-Qin1 BAO Shi-Xiang1*
(1. Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan 571101, China)
(2. Hainan University, Haikou, Hainan 570228, China)
(3. Hainan Zhengweier biotechnology Co., Ltd., Haikou, Hainan 571101, China)
Abstract: Strain DB09208, an endophytic bacterium exhibited strong inhibitory activity in vitro against Fusarium oxysporum f. sp. cubense FS-0704 (race 4: FOC4) was isolated from Musa AAA, cv. Baxi grown in Hainan. Based on the morphological, physiological and biochemical properties, and the 16S rDNA sequence analysis, this strain was identified as Paenibacillus sp.. The strain DB09208 obviously inhibited the mycelial growth of FS-0704, with an inhibitory rate of 74.09%, pot experiments indicated the strain suppressed Fusarium wilt of bananas, the control efficiency reached 65.2%. It could signifi-cantly promoted the generation of roots with the effect was similar to that of IAA. These demonstrated that strain DB09208 should be useful in biological control bananas Fusarium wilt.
Keywords: Fusarium wilt, Endophytic bacteria, 16S rDNA, Isolation and identification
