11月28日,，國際權(quán)威雜志《環(huán)境微生物學(xué)》Environment Microbiology刊登了浙江大學(xué)生命科學(xué)學(xué)院微生物研究所馮明光教授實驗室的兩項科研成果；第一項研究成果是馮明光教授發(fā)表了題為“Primary roles of two dehydrogenases in the mannitol metabolism and multi-stress tolerance of entomopathogenic fungus Beauveria bassiana”的研究論文,，在文中馮明光教授實驗室采用基因敲除與回補的方法,，成功解析了球孢白僵菌的兩種脫氫酶的生物學(xué)功能，發(fā)現(xiàn)這兩種酶在該菌的甘露醇代謝與多脅迫抗逆反應(yīng)中起著關(guān)鍵作用,。
另一成果是馮明光教授發(fā)表了題為“A Group III histidine kinase (mhk1) upstream of high-osmolarity glycerol pathway regulates sporulation, multi-stress tolerance and virulence of Metarhizium robertsii, a fungal entomopathogen"的研究論文,，馮明光教授實驗室利用相同方法（基因敲除與回補），成功解析了在羅伯茨綠僵菌高滲甘油代謝途徑上游的組氨酸激酶是調(diào)節(jié)該菌生長發(fā)育,、多脅迫抗性和毒力等重要性狀的關(guān)鍵激酶,，并證明它控制著其下游一系列蛋白激酶和表型相關(guān)性狀的基因表達。
這兩項研究成果均發(fā)表在《環(huán)境微生物學(xué)》Environmental Microbiology雜志上,，該雜志影響因子為5.638（IF 5.638 2010）,。（生物谷Bioon.com）
doi:10.1111/j.1462-2920.2011.02654.x
PMC:
PMID:
Primary roles of two dehydrogenases in the mannitol metabolism and multi-stress tolerance of entomopathogenic fungus Beauveria bassiana.
Wang ZL, Lu JD, Feng MG
Knockout and complement mutants of mannitol-1-phosphate dehydrogenase (MPD) and mannitol dehydrogenase (MTD) were constructed to probe the roles of both enzymes in the mannitol metabolism and multi-stress tolerances of entomopathogenic fungus Beauveria bassiana. Compared with wild-type and complement mutants, ΔBbMPD lost 99.5% MPD activity for reducing fructose-6-phosphate to mannitol-1-phosphate while ΔBbMTD lost 78.9% MTD activity for oxidizing mannitol to fructose. Consequently, mannitol contents in mycelia and conidia decreased 68% and 83% for ΔBbMPD, and 16% and 38% for ΔBbMTD, accompanied by greatly enhanced trehalose accumulations due to 81-87% decrease in their neutral trehalase expression. Mannitol as mere carbon source in a nitrate-based minimal medium suppressed the colony growth of ΔBbMTD instead of ΔBbMPD, and delayed more conidial germination of ΔBbMTD than ΔBbMPD. Based on median lethal responses, conidial tolerances to H(2) O(2) oxidation, UV-B irradiation and heat stress at 45°C decreased 38%, 39% and 22% in ΔBbMPD, and 18%, 16% and 11% in ΔBbMTD respectively. Moreover, ΔBbMPD and ΔBbMTD lost 14% and 7% of their virulence against Spodoptera litura larvae respectively. Our findings highlight the primary roles of MPD and MTD in mannitol metabolism and their significant contributions to multi-stress tolerances and virulence influential on the biocontrol potential of B. bassiana
