生物谷報(bào)道：今天剛剛出版的Nature上報(bào)道了有關(guān)干細(xì)胞的最新進(jìn)展，這一進(jìn)展意義重大，希望能閱讀
A basic doctrine of reproductive biology is that most mammalian females lose the capacity for germ-cell renewal during fetal life, such that a fixed reserve of germ cells (oocytes) enclosed within follicles is endowed at birth. Here we show that juvenile and adult mouse ovaries possess mitotically active germ cells that, based on rates of oocyte degeneration (atresia) and clearance, are needed to continuously replenish the follicle pool. Consistent with this, treatment of prepubertal female mice with the mitotic germ-cell toxicant busulphan eliminates the primordial follicle reserve by early adulthood without inducing atresia. Furthermore, we demonstrate cells expressing the meiotic entry marker synaptonemal complex protein 3 in juvenile and adult mouse ovaries. Wild-type ovaries grafted into transgenic female mice with ubiquitous expression of green fluorescent protein (GFP) become infiltrated with GFP-positive germ cells that form follicles. Collectively, these data establish the existence of proliferative germ cells that sustain oocyte and follicle production in the postnatal mammalian ovary.
Original article:
Germline stem cells and follicular renewal in the postnatal mammalian ovary145
JOSHUA JOHNSON, JACQUELINE CANNING, TOMOKO KANEKO, JAMES K. PRU & JONATHAN L. TILLY
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