生物谷報(bào)道：今天剛剛出版的Nature報(bào)道，證明了Myocardin 和ternary complex factors 共同競(jìng)爭(zhēng)SRF從而控制平滑肌的基因表達(dá)。
Smooth muscle cells switch between differentiated and proliferative phenotypes in response to extracellular cues1, but the transcriptional mechanisms that confer such phenotypic plasticity remain unclear. Serum response factor (SRF) activates genes involved in smooth muscle differentiation and proliferation by recruiting muscle-restricted cofactors, such as the transcriptional coactivator myocardin, and ternary complex factors (TCFs) of the ETS-domain family, respectively2-9. Here we show that growth signals repress smooth muscle genes by triggering the displacement of myocardin from SRF by Elk-1, a TCF that acts as a myogenic repressor. The opposing influences of myocardin and Elk-1 on smooth muscle gene expression are mediated by structurally related SRF-binding motifs that compete for a common docking site on SRF. A mutant smooth muscle promoter, retaining responsiveness to myocardin and SRF but defective in TCF binding, directs ectopic transcription in the embryonic heart, demonstrating a role for TCFs in suppression of smooth muscle gene expression in vivo. We conclude that growth and developmental signals modulate smooth muscle gene expression by regulating the association of SRF with antagonistic cofactors.
Myocardin and ternary complex factors compete for SRF to control smooth muscle gene expression 185
ZHIGAO WANG, DA-ZHI WANG, DIRK HOCKEMEYER, JOHN MCANALLY, ALFRED NORDHEIM & ERIC N. OLSON
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