（圖片來源：Lothar Schermelleh and Peter Carlton）
生物谷報道：科學家發(fā)明一種新型的顯微鏡能夠展示高清晰度,、多色彩的三維畫面,，它比以前用的傳統(tǒng)顯微鏡能揭示出更多的細節(jié),。這種技術叫做三維結構照明（3D-SIM）的新成像技術，并用它在大約100納米的分辨率下來探索哺乳動物的細胞核,。
最好的傳統(tǒng)顯微鏡的分辨率可以放大至200-300納米，但是研究人員利用這一新型的3D-SIM來捕捉多色彩,、三維畫面的細胞特征,，他們甚至能夠給細胞不同的成份標記上不同的顏色,，這是一個前所未有的壯舉,。這一新的發(fā)展使得分子細胞生物學有了令人感興趣的新的視角，而它又不需要任何非常規(guī)的設備,，并且也并不比一臺標準的商業(yè)用顯微鏡更難使用,。（生物谷www.bioon.com）
生物谷推薦原始出處：
Science，Vol. 320. no. 5881, pp. 1332 - 1336,，Lothar Schermelleh,，John W. Sedat
Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
Lothar Schermelleh,1* Peter M. Carlton,2* Sebastian Haase,2,4 Lin Shao,2 Lukman Winoto,2 Peter Kner,2 Brian Burke,3 M. Cristina Cardoso,4 David A. Agard,2 Mats G. L. Gustafsson,5 Heinrich Leonhardt,1* John W. Sedat2*
Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.
1 Center for Integrated Protein Science, Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany.
2 Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA.
3 Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610, USA.
4 Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.
5 Department of Physiology and Program in Bioengineering, University of California, San Francisco, CA 94143, USA.
