德韓兩國(guó)科學(xué)家8月28日在《自然》雜志上發(fā)表論文稱,，他們利用單個(gè)轉(zhuǎn)錄因子成功將人類干細(xì)胞回復(fù)到胚胎干細(xì)胞狀態(tài),。
今年早些時(shí)候，德國(guó)馬普分子生物醫(yī)學(xué)研究所的Hans Schöler和研究小組利用單個(gè)轉(zhuǎn)錄因子OCT4,，成功將小鼠成體神經(jīng)干細(xì)胞回復(fù)到多能狀態(tài),，能夠產(chǎn)生其他類型細(xì)胞，包括生殖細(xì)胞,。如今Schöler發(fā)現(xiàn),，OCT4同樣能在人類細(xì)胞中誘導(dǎo)多能性。
研究人員利用攜帶單一人類OCT4或OCT4和KLF4的逆轉(zhuǎn)錄酶病毒感染人類胎兒神經(jīng)干細(xì)胞（OCT4和KLF4是日本科學(xué)家山中伸彌于2006年最初進(jìn)行iPS細(xì)胞誘導(dǎo)的4個(gè)因子中的2個(gè)）,。感染后10到11周,，研究人員在兩種實(shí)驗(yàn)條件下均觀察到了類似于人類胚胎干細(xì)胞的細(xì)胞培養(yǎng)。
進(jìn)一步的檢查顯示,，這些細(xì)胞表達(dá)人類胚胎干細(xì)胞標(biāo)記,，而且全基因表達(dá)類似于人類胚胎干細(xì)胞。在試管中,，對(duì)多能性的嚴(yán)格測(cè)試證實(shí)這些細(xì)胞能夠形成所有3種胚層,。在活體內(nèi)，這些細(xì)胞能夠形成多種類型細(xì)胞,，包括呼吸上皮細(xì)胞,、骨骼肌細(xì)胞、軟骨細(xì)胞以及神經(jīng)上皮細(xì)胞,。
哈佛醫(yī)學(xué)院麥克林醫(yī)院干細(xì)胞研究人員Kwang-Soo Kim說(shuō)：“這是（干細(xì)胞）研究中另一個(gè)重要的里程碑,。”
美國(guó)斯克里普斯研究所的Sheng Ding表示：“證明OCT4足夠用于人類細(xì)胞類型重組是一個(gè)重大的進(jìn)步,，進(jìn)一步證實(shí)了早期在小鼠身上的類似發(fā)現(xiàn)。”（生物谷Bioon.com）
生物谷推薦原始出處：
Nature advance online publication 28 August 2009 | doi:10.1038/nature08436
Direct reprogramming of human neural stem cells by OCT4
Jeong Beom Kim1, Boris Greber1, Marcos J. Araúzo-Bravo1, Johann Meyer2, Kook In Park3, Holm Zaehres1 & Hans R. Schöler1
1 Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, R?ntgenstrasse 20, 48149 Münster, NRW, Germany
2 Hannover Medical School, Department of Experimental Hematology, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
3 Department of Pediatrics, BK21 Project for Medical Sciences, Yonsei University College of Medicine, 250 Seongsanno, Seodaemoon-gu, Seoul 120-752, Korea
Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4)1, 2, 3, 4, 5, 6, 7. We previously reported that Oct4 alone is sufficient to reprogram directly adult mouse neural stem cells to iPS cells8. Here we report the generation of one-factor human iPS cells from human fetal neural stem cells (one-factor (1F) human NiPS cells) by ectopic expression of OCT4 alone. One-factor human NiPS cells resemble human embryonic stem cells in global gene expression profiles, epigenetic status, as well as pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human neural stem cells to pluripotency. One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.
