生物谷www.bioon.net 提要：中國農(nóng)業(yè)大學(xué)、北京生命科學(xué)研究院（NIBS),、深圳作物熱帶亞熱帶分子設(shè)計(jì)育種中心以及堪薩斯州立大學(xué)的研究者聯(lián)合報(bào)道了細(xì)菌毒性蛋白HopF2幫助病原細(xì)菌侵染植物宿主的機(jī)理,。該研究發(fā)表于6月22日，國際植物學(xué)頂級(jí)雜志Plant Cell上,。
引自NIBS官方新聞：效應(yīng)蛋白是丁香假單胞菌致病的關(guān)鍵武器,。細(xì)菌將這些蛋白“注射”到植物宿主細(xì)胞內(nèi)，干擾植物的免疫信號(hào)通路以及其它細(xì)胞活動(dòng),，使宿主易感,。研究發(fā)現(xiàn)效應(yīng)蛋白HopF2直接作用于擬南芥的激酶MAP kinase kinases（MKKs），并在后者的C末端Arg313飾以ADP-ribose,。突變體分析表明HopF2與MKK的作用對前者的毒性功能必不可少,。結(jié)果還揭示了MKK的C末端對其功能的重要作用。
本研究主要在北京生命科學(xué)研究所完成,，研究獲得了國家科技部863計(jì)劃和北京市科委的資助,。通訊作者唐曉艷博士為堪薩斯州立大學(xué)植物病理學(xué)副教授，同時(shí)在深圳作物熱帶亞熱帶分子設(shè)計(jì)育種中心從事研究工作,。北京生命科學(xué)研究所周儉民實(shí)驗(yàn)室 王宇婧,、李積鳳、侯書國,、王星瑋等參與了研究工作,。這是周儉民研究組繼4月報(bào)道了一類寄主胞質(zhì)內(nèi)受體類似激酶是植物天然免疫信號(hào)傳導(dǎo)的新的重要元件又一成果（詳細(xì)內(nèi)容：Cell Host & Microbe：PTI和ETI在進(jìn)化上相互關(guān)聯(lián)新證據(jù)）。（生物谷Bioon.net）
急需英才：北京生命科學(xué)研究所招聘周儉民實(shí)驗(yàn)室招聘職位
生物谷推薦原文出處：
The Plant Cell DOI:10.1105/tpc.110.075697
A Pseudomonas syringae ADP-Ribosyltransferase Inhibits Arabidopsis Mitogen-Activated Protein Kinase Kinases
Yujing Wang a,b, Jifeng Li b, Shuguo Hou b, Xingwei Wang b, Yuan Li c, Dongtao Ren c, She Chen b, Xiaoyan Tang d,e,1 and Jian-Min Zhou b
a College of Life Sciences, Beijing Normal University, Beijing 100875, China
b National Institute of Biological Sciences, Beijing 102206, China
c State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
d Shenzhen Molecular Crop Design Center for Tropical and Subtropical Regions, Shenzhen 518040, China
e Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506
The successful recognition of pathogen-associated molecular patterns (PAMPs) as a danger signal is crucial for plants to fend off numerous potential pathogenic microbes. The signal is relayed through mitogen-activated protein kinase (MPK) cascades to activate defenses. Here, we show that the Pseudomonas syringae type III effector HopF2 can interact with Arabidopsis thaliana MAP KINASE KINASE5 (MKK5) and likely other MKKs to inhibit MPKs and PAMP-triggered immunity. Inhibition of PAMP-induced MPK phosphorylation was observed when HopF2 was delivered naturally by the bacterial type III secretion system. In addition, HopF2 Arg-71 and Asp-175 residues that are required for the interaction with MKK5 are also necessary for blocking MAP kinase activation, PAMP-triggered defenses, and virulence function in plants. HopF2 can inactivate MKK5 and ADP-ribosylate the C terminus of MKK5 in vitro. Arg-313 of MKK5 is required for ADP-ribosylation by HopF2 and MKK5 function in the plant cell. Together, these results indicate that MKKs are important targets of HopF2.
