近日,，日本國立東京醫(yī)科齒科大學(xué)的研究人員在英國期刊《自然·醫(yī)學(xué)》（Nature Medicine）上發(fā)表論文說,，他們通過在體外培養(yǎng)能發(fā)育成各種大腸組織的干細(xì)胞并進(jìn)行移植,，成功修復(fù)了小鼠的大腸潰瘍,。
2012杭州干細(xì)胞春季 會 議
來自東京醫(yī)科齒科大學(xué)的研究人員介紹說,，他們采集了小鼠大腸內(nèi)側(cè)表面的上皮組織,，然后利用特殊方法進(jìn)行培養(yǎng),，成功地大量增殖了其中的干細(xì)胞,。隨后，他們在患有大腸潰瘍的小鼠消化道末端注入干細(xì)胞,。結(jié)果,，這些干細(xì)胞只吸附在出現(xiàn)潰瘍的大腸部位。一個月后,，潰瘍處組織獲得再生,，潰瘍仿佛被蓋住似地消失了。
研究小組還利用相同方法,，對取自人體大腸組織的干細(xì)胞進(jìn)行培養(yǎng),，也獲得了成功。專家認(rèn)為,，如能進(jìn)一步完善相關(guān)技術(shù),，將有望開發(fā)出治療人體胃腸道疾病的新方法。（生物谷Bioon.com）
doi:10.1038/nm.2695
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Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell
Shiro Yui, Tetsuya Nakamura, Toshiro Sato, Yasuhiro Nemoto, Tomohiro Mizutani, Xiu Zheng, Shizuko Ichinose, Takashi Nagaishi, Ryuichi Okamoto, Kiichiro Tsuchiya, Hans Clevers & Mamoru Watanabe
Adult stem-cell therapy holds promise for the treatment of gastrointestinal diseases. Here we describe methods for long-term expansion of colonic stem cells positive for leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5+ cells) in culture. To test the transplantability of these cells, we reintroduced cultured GFP+ colon organoids into superficially damaged mouse colon. The transplanted donor cells readily integrated into the mouse colon, covering the area that lacked epithelium as a result of the introduced damage in recipient mice. At 4 weeks after transplantation, the donor-derived cells constituted a single-layered epithelium, which formed self-renewing crypts that were functionally and histologically normal. Moreover, we observed long-term (>6 months) engraftment with transplantation of organoids derived from a single Lgr5+ colon stem cell after extensive in vitro expansion. These data show the feasibility of colon stem-cell therapy based on the in vitro expansion of a single adult colonic stem cell.
