生物谷報(bào)道：本周Science報(bào)道細(xì)胞分裂中，中心粒凝聚的機(jī)理,，發(fā)現(xiàn)Sgo1, Chl4和Iml3這三個(gè)基因在其中起取關(guān)鍵性作用,，這一機(jī)理被揭示，加深了對(duì)細(xì)胞分裂機(jī)理的理解,，為研究新型藥物控制細(xì)胞周期過(guò)程提供理論依據(jù),。
A Genome-Wide Screen Identifies Genes Required for Centromeric Cohesion
Science,2004,29 Jan.
Adele L. Marston 1, Wai-Hong Tham 1, Hiral Shah 1, Angelika Amon 1*
1 Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
* To whom correspondence should be addressed. E-mail: [email protected].
During meiosis, two chromosome segregation phases follow a single round of DNA replication. We identified factors required to establish this specialized cell cycle by examining meiotic chromosome segregation in a collection of yeast strains lacking all non-essential genes. This analysis revealed Sgo1, Chl4 and Iml3 to be important for retaining centromeric cohesin until the onset of anaphase II. Consistent with this role, Sgo1 localizes to centromeric regions but dissociates at the onset of anaphase II. The screen described here provides a comprehensive analysis of the genes required for the meiotic cell cycle and identified three factors important for the stepwise loss of sister-chromatid cohesion.
