先將目標RNA分子插入到天然的tRNA支架上,，這種雜合的RNA既可以避免RNase的酶水解，又可以高保真地復制出大量的RNA分子,。
生物谷報道：一項在體內(nèi)表達功能性RNA分子的方法,，為簡化RNA樣品的準備打開了一扇方便之門，也為體外,、體內(nèi)研究RNA結(jié)構(gòu),、相互作用和功能提供了一套有用工具。
RNA新地位的發(fā)現(xiàn),，大大增加了RNA研究的重要性,。進一步加深對不同種類RNA的理解，甚至將之開發(fā)成為潛在的藥物靶點,，都需要在體內(nèi),、體外綜合研究RNA的結(jié)構(gòu)和功能。但是,，在這點上恰恰是當前研究所欠缺的,，部分原因在于缺乏合適的RNA實驗材料。一項新的方法——運用tRNA支架（tRNA scaffold）,，可在體內(nèi)大量表達RNA分子,，為解決此類問題提供了一條非常有借鑒價值的解決方案。
部分英文原文：
Nature Methods - 4, 571 - 576 (2007)
Published online: 10 June 2007; | doi:10.1038/nmeth1058
Recombinant RNA technology: the tRNA scaffold
Luc Ponchon & Frédéric Dardel
Cristallographie & RMN Biologiques, Université Paris Descartes, CNRS, 4 avenue de l'Observatoire, 75006, Paris, France.
Correspondence should be addressed to Frédéric Dardel frederic.dardel@univ-paris5.fr
RNA has emerged as a major player in most cellular processes. Understanding these processes at the molecular level requires homogeneous RNA samples for structural, biochemical and pharmacological studies. So far, this has been a bottleneck, as the only methods for producing such pure RNA have been in vitro syntheses. Here we describe a generic approach for expressing and purifying structured RNA in Escherichia coli, using tools that parallel those available for recombinant proteins. Our system is based on a camouflage strategy, the 'tRNA scaffold', in which the recombinant RNA is disguised as a natural RNA and thus hijacks the host machinery, escaping cellular RNases. This opens the way to large-scale structural and molecular investigations of RNA function.
